气相色谱用于肠通透性监测的研究

作者采用国产气相色谱及９２０２微机数据处理系统，对临床患者和实验动物肠通透性改变进行监测。该方法以甘露醇（Ｍ）和乳果糖（Ｌ）为探针，用气相色谱检测尿中糖分泌率及Ｌ／Ｍ比值。结果显示，测定标准品Ｍ和Ｌ随进样量增加而呈线性改变，与国外学者报道一致。比较不同浓度进口与国产乳果糖，证明二者在研究肠通透性方面高度相关（ｒ＝０．９９）。检测急性胰腺炎并发感染动物的尿标本，发现乳果糖大量排出，Ｌ／Ｍ值明显增加。作者认为，气相色谱为一行之有效的方法用于肠通透性监测，有助于临床上对内源性感染及脓毒症的早期诊断。

Measurement of urinary content of lactulose and mannitol by gas chromatography as an index of permeability of the gut

We established lactulose mannitol(L M) measurement method by gas chromatography and 9202 computer data processing system to test intestinal permeability.The urine output of L M was in linear correlation to its sample concentration within working range. In an animal model of acute pancreatitis, lactulos secretion increased in urine, together with increased L/M ratio.The measurement of lactulose mannitol intestinal permeability by our method might serve as a predictor for early diagnosis of endogenous infection and sepsis.