A feeding strategy for tetramethylpyrazine production by Bacillus subtilis based on the stimulating effect of ammonium phosphate |
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Authors: | Bing-Feng Zhu Yan Xu |
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Affiliation: | (1) State Key Laboratory of Food Science and Technology, Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Avenue, Wuxi, 214122, China; |
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Abstract: | To examine the effects of ammonium salts on tetramethylpyrazine (TTMP) production by Bacillus subtilis CCTCC M 208157, different ammonium salts were tested, and diammonium phosphate (DAP) was found to have a predominant effect
on stimulating TTMP synthesis. The DAP requirements for TTMP production were then investigated, experimental results showed
that higher concentrations of DAP favored TTMP production, while both the ammonium and phosphate ions exhibited inhibitory
effects on the cell growth and precursor 3-hydroxy-2-butanone accumulation. Based on the results above, a DAP feeding strategy
was developed and verified in further experiments. By applying the proposed fed-batch strategy, the maximum TTMP concentrations
reached 7.46 and 7.34 g/l in flask and fermenter experiments, increased by 55.1 and 29.0% compared to that of the batch TTMP
fermentation, respectively. To our knowledge, these results, i.e., TTMP yields in flask or fermenter fermentations, were new
records on TTMP fermentation by B. subtilis. |
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Keywords: | |
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