头花蓼不同地理居群的叶绿体psbA-trnH和 trnL-trnF基因序列与聚类分析

目的:探讨民族药头花蓼居群间的DNA序列变异与地理分布的关系,为头花蓼优良种质资源的筛选评价提供分子依据.方法:采用PCR产物直接测序,对头花蓼11个居群11个个体样品的叶绿体psbA-trnH,trnL-trnF基因进行测序分析研究.结果:头花蓼11个居群的叶绿体psbA-trnH长402 bp,存在6个变异位点.trnL-trnF长875 bp,存在5个变异位点.用UPGMA法构建的聚类图表明,头花蓼在贵州至云南的类群存在较大程度变异.结论:贵州西部与云南东部地区的头花蓼有利于优良种质资源的筛选.

Analysis on chloroplast DNA sequences of Polygonum capitatum of different geographical population

Objective : To investigate the relationship between the variation of chloroplast DNA gene sequences and the geographical origins of Polygonum capitatum in order to provide the molecular evidence for its excellent germplasm resources. Method : PCR direct sequencing was applied to detect the chloroplast psbA-trnH, trnL-trnF gene sequence of 11 samples collected from 11 populations of P. capitatum. Result : The psbA-trnH gene sequence of P. capitatum from different populations was 402 bp in length, there were 6 variable sites. TrnL-F gene sequence was 875 bp, there were 5 variable sites. The clusters diagram by UPGMA method showed that P. capitatum groups in Yunnan and Guizhou existed a considerable variation. Conclusion : P. capitatum which is located in the east of Yunnan and the west of Guizhou is helpful of screening the germplasm resources.