| 人肝癌P57~(kip2)基因失表达的遗传不稳定性 |
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| 引用本文： | 易晓佳,申丽娟,章宗籍.人肝癌P57~(kip2)基因失表达的遗传不稳定性[J].基础医学与临床,2010,30(3):272-274. |
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| 作者姓名： | 易晓佳 申丽娟 章宗籍 |
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| 作者单位： | 昆明医学院,病理教研室,云南,昆明,650031 |
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| 基金项目： | 云南省自然科学基金(2005C0045M) |
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| 摘 要： | 目的探讨肝细胞癌(HCC)P57kip2mRNA表达与遗传不稳定性之间的相互关系,以探索P57kip2基因失表达的机制。方法用原位分子杂交技术检测肝癌组织中P57kip2mRNA表达,用PCR-聚丙烯酰胺凝胶电泳-银染法检测LOH和MSI。结果在正常肝组织未见P57kip2mRNA表达,癌周肝硬化组与肝癌组阳性表达率均为26.7%(8/30)。3个位点均未发生LOH;在2个微卫星位点发生MSI,MSI总阳性率为16.7%。D11S1760位点发生MSI与P57kip2mRNA表达有关联性(P<0.05)。结论P57kip2mRNA表达异常提示其可能在HCC发生过程中起重要作用。MSI可能是肝癌发生过程中P57kip2mRNA表达异常的原因之一。
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| 关 键 词： | 肝癌 P57kip2 遗传不稳定性 杂合性缺失 微卫星不稳定性 |
| 收稿时间： | 2009-2-20 |
| 修稿时间： | 2009-7-9 |
Genetic instability of P57~(kip2) misexpression in human hepatocellular carcinoma |
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| YI Xiao-jia,SHEN Li-juan,ZHANG Zong-ji.Genetic instability of P57~(kip2) misexpression in human hepatocellular carcinoma[J].Basic Medical Sciences and Clinics,2010,30(3):272-274. |
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| Authors: | YI Xiao-jia SHEN Li-juan ZHANG Zong-ji |
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| Affiliation: | Dept.of Pathology;Kunming Medical College;Kunming 650031;China |
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| Abstract: | Objective To study the expressions of P57kip2 mRNA and genetic instability of P57kip2 in human hepatocellular carcinoma (HCC).Methods In situ hybridization(ISH) was used to detect the expression of P57kip2 mRNA in HCC.MSI and LOH were detected by PCR-polyacrylamide gel electrophoresis-silver staining method.Results There was no expression of P57kip2 mRNA found in normal liver tissue.The expression rate of P57kip2 mRNA in both pericancerous cirrhosis and hepatocellular carcinoma was 26.7%(8/30).LOH was not i... |
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| Keywords: | HCC P57kip2 genetic instability LOH MSI |
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