机器人辅助腹腔镜前列腺癌根治术(RALP)与开放性耻骨后前列腺癌根治术(RRP)的5年肿瘤学效果对比

 目的 分析机器人辅助腹腔镜前列腺癌根治术（robot-assisted laparoscopic radical prostatectomy，RALP）与开放性耻骨后前列腺癌根治术（open retropubic radical prostatectomy，RRP）治疗局限性前列腺癌的5年肿瘤学效果。方法 收集复旦大学附属华东医院泌尿外科2009年7月至2014年3月同期接受RALP或RRP并获得随访的95例患者临床资料，比较两种术式的手术时间、术中失血量及输血比例、术后病理、生化复发率等指标。结果 95例手术均获成功，RALP组无中转开放。RALP组与RRP组手术时间分别为212.3与166.8 min（P<0.05），术中失血量分别为237.3与557.3 mL（P<0.05）；术中输血比例分别为16.7%与78.7%（P<0.05）；手术切缘阳性率分别为22.0%与7.8%（P<0.05），1年尿控率分别为79.5%和84.7%，6个月、1年、2年、3年、4年、5年生化复发率分别为6.2%和4.3%、18.8%和6.4%、25.0%和14.9%、29.2%和21.3%、31.2%和21.3%、31.2%和23.4%。两组5年内的生化复发率差异无统计学意义。结论 RALP具有损伤小、出血少、输血少的优点，但手术时间长于RRP。两种术式的尿控、手术切缘阳性率及5年长期肿瘤学效果等相似。

The role of urokinase type plasminogen activator gene in mouse experimental emphysema

Objective To observe the role of urokinase type plasminogen activator (u-PA) gene in mice experimental senile emphysema changes and its possible mechanism. Methods Wild type (WT) mice and u-PA gene knock out (KO) mice were fed under the criterion of SPF. At 15th day and 4th month, mice were anaesthetised and anatomized for further experiments. Part of mice lung was prepared for HE and weigert staining, another parts of lung were picked for real- time PCR. Results 15th day old WT and u-PA gene KO mice had similar lung alveolar structure. The content of elastin, elastin mRNA, MMP-12 mRNA in the lung had no significant difference between these two genotype mice. With aging, 4th month old u-PA gene KO mice had more integrated alveolar cavity. The elastin appears to be higher than that of WT mice. The elastin and TIMP-1 gene expression levels increased greatly in u-PA gene KO mice. Conclusions Disruption of u-PA gene might protect the lung alveolar structure through increasing elastin by enhancing the elastin and TIMP-1 gene expression.