左卡尼汀对心肺复苏术后大鼠脑神经细胞氧自由基损伤的保护作用

目的]通过观察左卡尼汀大鼠对心肺复苏后脑组织丙二醛(MDA)、超氧化物歧化酶(SOD)含量及神经细胞凋亡的变化,探讨左卡尼汀对复苏后脑神经细胞保护作用的机制.方法]建立心肺复苏大鼠模型,90只Wistar大鼠随机分为手术对照组(S组)、常规复苏组(C组)和左卡尼汀组 (L组),每组30只,S组只行手术操作,不致颤;...

Protective Effect of Levocarnitine on Oxygen Radical Injury of Brain Nerve Cells in Rats after Cardiopulmonary Resuscitation

Objective] To observe the changes of malondialdehyde （ MDA）, superoxide dismutase （SOD） and nerve cell apoptosis of brain tissue in rats treated by levocarnitine after cardiopulmonary resuscitation （CPR） and explore the mechanism of neuroprotective effects of levocarnitine. Methodsl Rat model of CPR was established. Ninety Wistar rats were randomly divided into operation-control group（group S, n = 30）, routine resuscitation group（group C, n =30） and levocarnitine group（group L, n =30）. Group S was only performed the operation but not induced cardiac arrest. Group C was performed standard CPR after cardiac arrest. Group L was given intravenous injection of levocarnitine after CPR of cardiac arrest. The changes of MDA, SOD and apoptosis in nerve cells of gyrus hippocampi were observed dynamically. HE staining was used to observe the pathological change of brain tissue in rats. ResultsI Compared with group S, MDA and the number of apoptotic cells in brain tissue after CPR gradually increased and the level of SOD in brain tissue gradually decreased in group C and group L（all P （0.01）. Compared with group C at the same stage, the in- creasing of MDA and apoptotic ceils in brain tissue alleviated（ P 〈0.05）, and the decreasing of SOD in brain tissue reduced（ P 〈0.05）, and brain damage under light microscope relieved in group L. ConclusionlLevoc-arnitine has the neuroprotective effect against oxygen radical injury of brain nerve cells after CPR. It may be related to the ability of levocarnitines for increasing the antioxidase activity and inhibiting the production of oxygen radicals and lipid peroxidation in brain tissue.