肉桂醛对链格孢菌生长及非寄主选择性毒素合成的影响

目的 初步探究肉桂醛对葡萄采后链格孢菌菌丝体生长及非寄主选择性毒素合成的影响。方法 采用扫描电镜(scanning electron microscopy, SEM)观察肉桂醛处理后链格孢菌菌丝体的形态结构; 测定处理后菌丝体脂质和麦角固醇含量, 胞外电导率、OD260值和菌丝体荧光强度; 高效液相色谱(high performance liquid chromatography, HPLC)测定处理后链格孢酚(alternariol, AOH)、交链格孢酚单甲醚(alternariol monomethyl ether, AME)含量的变化。结果 扫描电镜结果显示肉桂醛处理后链格孢菌菌丝体形态结构明显被破坏; 脂质和麦角固醇含量显著下降; 胞外电导率和OD260值以及菌丝体荧光强度显著增加; AOH、AME的含量显著下降。 结论 肉桂醛通过破坏细胞膜通透性和完整性来抑制链格孢菌菌丝体生长, 同时体内主要非寄主选择性毒素合成受到抑制。

Effects of cinnamaldehyde on growth and the synthesis of non-host specific toxins of Alternaria alternata

Objective To investigate the effects of cinnamaldehyde on growth and the synthesis of non-host specific toxins of Alternaria alternata. Methods The morphology of A. alternata mycelium treated with cinnamaldehyde was observed by scanning electron microscopy (SEM). The contents of lipids and ergosterol, extracellular conductivity, OD260 value and mycelial fluorescence intensity were determined after treatment. The content variation of alternariol (AOH) and alternariol monomethyl ether (AME) were measured by high performance liquid chromatography (HPLC). Results SEM showed that the morphology and structure of A. alternata were destroyed after treated with cinnamaldehyde. The contents of lipids, ergosterol, AOH and AME decreased significantly, while the extracellular conductivity, OD260 value and mycelial fluorescence intensity increased significantly. Conclusion Cinnamaldehyde inhibited mycelial growth by destroying permeability and integrity of cell membrane, meanwhile, the synthesis of non-host specific toxins was inhibited in vivo.