高效液相色谱法同时测定枣果实中的有机酸和VC含量

目的:建立枣果实中有机酸含量的高效液相色谱测定方法。方法:采用高效液相色谱-二极管阵列检测器法,水浴超声、离心、膜过滤提取枣果实中的有机酸。色谱条件:色谱柱为Inertsil ODS-3(4.6 mm×250 mm,5 μm),流动相为0.04 mol/m L KH_2PO_4溶液,流速0.5 m L/min,柱温30℃,检测波长210 nm。结果:在此色谱条件下,草酸、酒石酸、奎宁酸、苹果酸、柠檬酸、富马酸和VC在30 min之内得到基线分离。7种有机酸和VC标准曲线的相关系数(R2)均在0.998 9以上;精密度检测,相对标准偏差为0.98%~4.77%(n=5);重复性检测,相对标准偏差为0.88%~2.19%(n=5);回收率在91.35%~105.74%之间。结论:本方法准确、高效、便捷,适于枣果实中有机酸的测定。

Simultaneous Determination of Organic Acids and Vitamin C in Jujube Fruits by HPLC

Objective: This study aimed to establish and validate a high performance liquid chromatographic (HPLC) method
for the fast simultaneous determination of organic acids and vitamin C (VC) in jujube fruits. Methods: The analytes in
samples were extracted ultrasonically in a water bath, centrifugated and purified by membrane filtration for analysis by
HPLC with diode-array detection (DAD). The chromatographic column used was Inertsil ODS-3 (4.6 mm × 250 mm, 5 μm).
The separation was performed using 0.04 mol/L KH2PO4 (pH 2.40) as mobile phase at 0.5 mL/min flow rate by isocratic
elution. The column temperature and detection wavelength were set as 30 ℃ and 210 nm, respectively. Results: Under
these chromatographic conditions, 7 organic acids and VC could be successfully separated in 30 minutes. The coefficients
of determination (R2) of the standard calibration curves of the analyzed compounds were all above 0.998 9 and the precision
expressed as relative standard deviations were between 0.98% and 4.77% (n = 5) and the repeatability RSDs were in the
range of 0.88% to 2.19% (n = 5). The mean recovery rates of seven organic acids and vitamin C were in the range of 91.35%
to 105.74%. Conclusion: This method is an accurate, rapid, and applicable method to determine organic acids and vitamin C
in jujube fruits.