| 血管紧张素转换酶抑制肽RLSFNP脂质体的制备工艺优化 |
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| 引用本文： | 郭宇星,薛逸秋,姜潇潇,吴振,曾小群,孙杨赢,潘道东.血管紧张素转换酶抑制肽RLSFNP脂质体的制备工艺优化[J].食品科学,2017,38(20):139-145. |
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| 作者姓名： | 郭宇星 薛逸秋 姜潇潇 吴振 曾小群 孙杨赢 潘道东 |
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| 作者单位： | （1.南京师范大学金陵女子学院，江苏?南京 210097；2.宁波大学海洋学院，浙江?宁波 315211） |
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| 基金项目： | 国家自然科学基金面上项目（31571852；31471598；31671869；31601487）；江苏省自然科学基金项目（BK20151544）；2017年江苏省第五期 “333工程”培养资金资助项目（BRA2017450） |
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| 摘 要： | 本实验以脂质体作为包埋载体,研究血管紧张素转换酶(angiotensin converting enzyme,ACE)抑制肽Arg-Leu-Ser-Phe-Asn-Pro(RLSFNP)脂质体的制备工艺。以包封率为考察指标,采用单因素试验和响应面试验优化RLSFNP脂质体的制备工艺。得出最优制备条件为:大豆卵磷脂用量120.0 mg、胆固醇用量24.6 mg、RLSFNP用量15.3 mg、乙醚用量15.0 m L、磷酸盐缓冲液(p H 7.4)用量5.8 m L、探头超声时间5.2 min。在此条件下制备的脂质体平均粒径在168 nm左右,电位为-31.2 m V,实际包封率为(67.5±0.8)%,由脂质体包埋后的RLSFNP具有一定的缓释效果。
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| 关 键 词： | 血管紧张素转换酶抑制肽 脂质体 包封率 生物利用率 |
Optimization of Preparation of Angiotensin Converting Enzyme (ACE) Inhibitory Peptide RLSFNP Liposomes |
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| GUO Yuxing,XUE Yiqiu,JIANG Xiaoxiao,WU Zhen,ZENG Xiaoqun,SUN Yangying,PAN Daodong.Optimization of Preparation of Angiotensin Converting Enzyme (ACE) Inhibitory Peptide RLSFNP Liposomes[J].Food Science,2017,38(20):139-145. |
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| Authors: | GUO Yuxing XUE Yiqiu JIANG Xiaoxiao WU Zhen ZENG Xiaoqun SUN Yangying PAN Daodong |
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| Affiliation: | (1. Jinling College, Nanjing Normal University, Nanjing 210097, China; 2. School of Marine Sciences, Ningbo University, Ningbo 315211, China) |
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| Abstract: | The objective of the present study was to optimize the preparation of liposomes incorporating the angiotensin converting enzyme (ACE) inhibitory peptide Arg-Leu-Ser-Phe-Asn-Pro (RLSFNP) using combination of one-factor-at-a-time method and response surface methodology. The response variable was the entrapement efficiency. The optimum preparation conditions were determined as follows: soy lecithin, 120.0 mg; cholesterol, 24.6 mg; RLSFNP, 15.3 mg; ether, 15 mL; PBS buffer (pH 7.4), 5.8 mL; and ultrasonication time, 5.2 min. Under these conditions, the average diameter, potential and entrapement efficiency of liposomes were 168 nm, –31.2 mV and (67.5 ± 0.8) %, respectively. The liposomes could sustainably release RLSFNP. |
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| Keywords: | angiotensin converting enzyme (ACE) inhibitory peptides liposomes entrapement efficiency bioavailability |
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