PCR-核酸试纸条法检测食品中假结核耶尔森菌

目的 建立一种基于PCR-核酸试纸条技术快速检测食品中假结核耶尔森菌的方法。方法 将10株假结核耶尔森菌株和9株其他耶尔森氏菌及18株来源菌株作为实验菌株进行特异性实验; 通过纯菌液计数、干扰菌实验检测进行灵敏度验证。结果 DNA检测可达到10?3 μg/mL, 25 g样品加菌实验灵敏度可达 100 CFU/25 g, 添加10倍干扰菌不会降低检测灵敏度。利用建立方法对市场购买的食品进行筛查并与国标方法进行比较, 建立方法的灵敏度优于国标方法。结论 该方法检测结果准确, 灵敏度高, 适用于检测食品中假结核耶尔森菌。

Detection of Yersinia pseudotuberculosis in food by PCR-nucleic acid strips method

Objective To establish a method for rapid determination of Yersinia pseudotuberculosis in food based on PCR-nucleic acid test strip technology. Methods Ten strains of Yersinia pseudotuberculosis, 9 other strains of Yersinia enterocolitica and 18 strains of source strains were used as test strains for specific tests. The sensitivity was verified by counting pure bacterial liquid and testing by interfering bacteria. Results DNA testing could be up to 10-3 min/mL, and 25 g samples could be tested for bacteria with a sensitivity of 100 CFU/25 g. Addition of 10 times of interfering bacteria would not reduce the detection sensitivity. Using the established method to screen the food purchased in the market and compare it with the standard method, the sensitivity of the established method was better than that of national standard method. Conclusion This method is accurate, sensitive and suitable for the detection of Pseudotuberculosis Yersinia in food.