| 龙葵果花色苷的分离与鉴定 |
| |
| 引用本文： | 腾 飞,郑 悦,王 萍.龙葵果花色苷的分离与鉴定[J].食品科学,2016,37(7):56-61. |
| |
| 作者姓名： | 腾 飞 郑 悦 王 萍 |
| |
| 作者单位： | 东北林业大学林学院，黑龙江 哈尔滨 150040 |
| |
| 摘 要： | 采用硅胶柱层析技术分离制备龙葵果花色苷。分离得到的2 个花色苷馏分经紫外-可见光谱、高效液相色谱-电喷雾串联质谱（high performance liquid chromatography electrosprary ionization-mass spectrometry，HPLC-DADESI-MS/MS）进行结构鉴定，并结合酸水解分析糖苷种类。最终确定馏分Ⅰ为飞燕草素-3-琥珀酰阿拉伯糖苷，根据峰面积归一化法计算其纯度为94%；馏分Ⅱ为矢车菊素-3-半乳糖苷和矢车菊素-3-乙酰半乳糖苷，峰面积归一化法计算其纯度分别为45.67%和13.97%。
|
| 关 键 词： | 龙葵 花色苷 分离 鉴定 高效液相色谱-电喷雾质谱联用 酸水解 |
Isolation and Identification of Anthocyanins from Solanum nigrum L. Fruits |
| |
| TENG Fei,ZHENG Yue,WANG Ping.Isolation and Identification of Anthocyanins from Solanum nigrum L. Fruits[J].Food Science,2016,37(7):56-61. |
| |
| Authors: | TENG Fei ZHENG Yue WANG Ping |
| |
| Affiliation: | College of Forestry, Northeast Forestry University, Harbin 150040, China |
| |
| Abstract: | The objective of this study was to isolate anthocyanins from Solanum nigrum L. fruits by silica gel column
chromatography and identify them by UV-visible spectroscopy and high performance liquid chromatography-electrospray
ionization tandem mass spectrometry (HPLC-DAD-ESI-MS/MS). Acid hydrolysis was applied for the identification
of glucosides. Three anthocyanins were identified for the first time in Solanum nigrum L. fruits. Only delphinidin-3-
succinylarabinoside was detected in fraction I (the purity was 94%), while both cyanidin-3-galactoside (the purity was
45.67%) and cyanidin-3-acetylgalactoside (the purity was 13.97%) were found to be present in fraction II. The presented
method is simple and reliable to isolate and identify the anthocyanins from Solanum nigrum L. fruits. |
| |
| Keywords: | Solanum nigrum L anthocyanins isolation identification high performance liquid chromatography electrosprary ionization-mass spectrometry (HPLC-DAD-ESI-MS/MS) acid hydrolysis |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《食品科学》浏览原始摘要信息 |
|
点击此处可从《食品科学》下载全文 |
