| 液相色谱-质谱法快速检测4种乳源低聚糖 |
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| 引用本文： | 魏京华,陈历俊,赵军英,张 毅,景萌娜,王 品,姜铁民,乔为仓.液相色谱-质谱法快速检测4种乳源低聚糖[J].食品科学,2016,37(14):86-91. |
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| 作者姓名： | 魏京华 陈历俊 赵军英 张 毅 景萌娜 王 品 姜铁民 乔为仓 |
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| 作者单位： | 1.大连工业大学生物工程学院，辽宁 大连 116000；;
2.北京三元食品股份有限公司国家母婴乳品健康工程技术研究中心，北京 100163 |
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| 基金项目： | “十二五”国家科技支撑计划项目（2013BAD18B04）；北京市科技计划项目（D141100004814002） |
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| 摘 要： | 建立一种乳样经简单预处理后采用超高效液相色谱-串联质谱同时检测4种重要的乳源低聚糖方法。牛乳及人乳经过脱脂、脱蛋白后经amide液相色谱柱用0.1%氨水-乙腈溶液梯度分离,采用质谱检测。2种唾液酸乳糖标准品的标准曲线在0.78~50.00 mg/L范围内线性良好(R~20.990),2种岩藻糖乳糖标准品的标准曲线在1.56~100.00 mg/L范围内线性良好(R~20.990)。加标回收率在80.00%~100.00%之间,重复性、精密度实验所得相对标准偏差均小于5.00%。
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| 关 键 词： | 超高效液相色谱-串联质谱 低聚糖 牛乳 人乳 |
Rapid and Simultaneous Detection of 4 Milk Oligosaccharides Using LC-MS |
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| WEI Jinghua,CHEN Lijun,ZHAO Junying,ZHANG Yi,JING Mengna,WANG Pin,JIANG Tiemin,QIAO Weicang.Rapid and Simultaneous Detection of 4 Milk Oligosaccharides Using LC-MS[J].Food Science,2016,37(14):86-91. |
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| Authors: | WEI Jinghua CHEN Lijun ZHAO Junying ZHANG Yi JING Mengna WANG Pin JIANG Tiemin QIAO Weicang |
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| Affiliation: | 1. School of Biological Engineering, Dalian Polytechnic University, Liaoning 116000, China;
2. National Maternal and Infant Health Dairy Researching Center of Beijing Sanyuan Foods Co. Ltd., Beijing 100163, China |
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| Abstract: | An ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was used to
rapidly and simultaneously detect 4 oligosaccharides in bovine milk and human milk. Samples were defatted, deproteined,
resolved on an ACQUITY BEH amide column using gradient elution with 0.1% ammonia water/acetonitrile as mobile
phase, detected by mass spectrometric specific ion monitoring and quantified. The standard curves of 2 fucosyllactose
oligosaccharides were linear within the range from 0.78 to 50.00 mg/L with R2 > 0.990. The standard curves of 2
sialyllactose were linear within the range from 1.56 to 100.00 mg/L with R2 > 0.990. The recoveries of spiked samples
ranged from 80.00% to 100.00%. The relative standard deviation (RSD) of repeatability and precision was less than 5.00%. |
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| Keywords: | ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) oligosaccharides bovine milk human milk |
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