花椒麻味物质分子印迹聚合物的制备及吸附特性

为实现对花椒麻味物质经济高效的纯化制备,本实验采用分子印迹技术,以甲基丙烯酸为功能单体,花椒麻味物质为模板分子,合成对花椒麻味物质具有特异性识别和吸附能力的分子印迹聚合物(molecularly imprinted polymers,MIPs),并用MIPs制成固相萃取柱用于分离纯化花椒油树脂中的花椒麻味物质。吸附动力学实验结果显示,MIPs与花椒麻味物质接触约12 h后就达到吸附平衡,对花椒麻味物质识别因子α达到3.05;等温吸附实验表明,MIPs对花椒麻味物质的吸附量高于空白聚合物,在吸附过程中模板分子花椒麻味物质与MIPs形成两种结合位点,两种结合位点的解离常数分别为1.172×10~(-2) mg/m L和3.585×10~(-2) mg/m L,对花椒麻味物质最大表观吸附量分别为11.133 mg/g和15.802 mg/g;固相萃取实验表明,该固相萃取柱可以分离出相对纯度为94.09%的花椒麻味物质。

Preparation and Binding Characteristics of Molecularly Imprinted Polymers for the Numb-Taste Components of Zanthoxylum bungeanum

In order to develop an economical and efficient approach to prepare purified numb-taste compounds from Zanthoxylum bungeanum, molecularly imprinted polymers (MIPs) were synthesized by bulk polymerization, using the numb-taste components of Zanthoxylumas bungeanum as the template molecule and methylacrylic acid (MAA) as the functional monomer in this work, and they were used to develop a solid phase extraction (SPE) column. The experimental results of adsorption kinetics showed that the adsorption of the numb-taste components on MIPs could reach equilibrium in 12 h and the recognition factor (α) was 3.05. The adsorption isothermal curves of MIPs and non-molecularly imprinted polymers (NIPs) showed that the adsorption quantity of the numb-taste components into the cavity formed by MIPs was higher than into that formed by NIPs. Two distinct binding sites were observed between the imprinted polymer and the template (the numb-taste components). The dissociation constants (Kd) for the binding sites were 1.172 × 10-2 and 3.585 × 10-2 mg/mL, and the apparent maximum adsorption capacity (Qmax) were 11.133 and 15.802 mg/g, respectively. The numb-taste compounds with 94.09% purity from Zanthoxylumas bungeanum were obtained using the MIP-based SPE column developed.