| 牙龈成纤维细胞和牙周韧带细胞前列腺素含量分析 |
| |
| 引用本文： | 陈铁楼,宋培智,吕王麟.牙龈成纤维细胞和牙周韧带细胞前列腺素含量分析[J].上海口腔医学,1995,4(3):155-157. |
| |
| 作者姓名： | 陈铁楼 宋培智 吕王麟 |
| |
| 作者单位： | 上海海军４１１医院口腔科牙周病室 |
| |
| 摘 要： | 作者对体外培养的人牙龈成纤维细胞（ＧＦ）和牙周韧带细胞（ＰＤＬＣ）采用免疫组化ＡＢＣ染色及显微分光光度法分析，测定ＧＦ和ＰＤＬＣ中前列腺素Ｅ２（ＰＧＥ２）、６－酮一前列腺素Ｆ１ａ．（６－Ｋ－ＰＧＦ１ａ）和血栓素Ｂ２（ＴＸＢ２）含量，以探讨其与牙周炎的关系，结果表明ＧＦ的ＰＧＥ２吸光度为０．２５±０．０３，６－Ｋ－ＰＧＦ１ａ的吸光度为０．２０±０．０３，与对照组相比均有非常显著性差异；ＰＤＬＣ的吸光度分别为ＰＧＥ２０．２１±０．０２、ＴＸＢ２０．１６±０．０３和６－Ｋ－ＰＧＦ１ａ０．１３±０．０２，与对照组相比均有非常显著性差异。表明ＧＦ和ＰＤＬＣ均可分泌一定量的ＰＧＥ２、ＴＸＢ２和６－Ｋ－ＰＧＦ１ａ，但分泌的量略有差异，提示ＧＦ和ＰＤＬＣ可通过分泌ＰＧ，影响牙龈炎症变化和牙周组织再生。
|
| 关 键 词： | 牙龈 成纤维细胞 牙周韧带 细胞前列腺素 免疫组化ABC法 显微分光光度法 牙周炎 |
Analysing the contents of prostaglandins in gingival fibroblast and periodontal ligament cell by microspectrophotometer] |
| |
| T L Chen,P Z Song,Y L Lu.Analysing the contents of prostaglandins in gingival fibroblast and periodontal ligament cell by microspectrophotometer][J].Shanghai Journal of Stomatology,1995,4(3):155-157. |
| |
| Authors: | T L Chen P Z Song Y L Lu |
| |
| Affiliation: | The Stomatological Research Center of Navy. Shanghai 200081,China. |
| |
| Abstract: | This investigation analyses the contents of prostaglandin E(2)(PGE(2)),6-keto-prostaglandin F(1a)(6-k-PGF(1a)) and thromboxane B(2)(TXB(2)) in gingival fibroblast (GF) and periodontal ligament cell(PDLC) by microspectrophotometer.The results showed that the absorbance of PGE(2) in GF was 0.25+/-0.03,the absorbance of 6-k-PGF(1a) was 0.20+/-0.03,there were significant difference between PGE(2),6-k-PGF(1a) and control group.The absorbance of PGE(2),TXB(2) and 6-k-PGF(1a) and control group.The absorbance of PGE(2),TXB(2) and 6-k-PGF(1a) in PDLC each were 0.20+/-0.20,0.16+/-0.03 and 0.13+/-0.02.They were much bigger than control group(0.07+/-0.01),which indicated prostaglandins in GF and PDLC can affect guided tissue regeneration and gingivitis degree,and there were important relationship between PG(s) in GF,PDLC and periodontitis. |
| |
| Keywords: | |
| 本文献已被 CNKI 等数据库收录！ |
|
