补益强心片对慢性心力衰竭大鼠的保护作用

目的研究补益强心片对慢性心力衰竭大鼠的保护作用。方法 60只大鼠随机分为正常对照组、假手术组、模型组及补益强心片低、中、高剂量组(0.18、0.36、0.72 g/kg),结扎心脏左冠状动脉前降支的方法建立慢性心力衰竭模型。灌胃给药4周后,超声仪检测血流动力学参数(LVEDd、LVESd、LVEF),ELISA检测BNP、ANP水平,流式细胞术检测细胞凋亡率,RT-PCR检测Bcl-2、Bax、caspase-3 mRNA表达,Western blot检测Bcl-2、Bax、caspase-3、PI3K、p-PI3K、Akt、p-Akt蛋白表达。结果与模型组比较,补益强心片组血流动力学参数显著改善(P<0.05,P<0.01),BNP、ANP水平,细胞凋亡率,Bax、caspase-3 mRNA和蛋白表达显著降低(P<0.01),Bcl-2 mRNA和蛋白表达、p-PI3K/PI3K、p-Akt/Akt显著升高(P<0.05,P<0.01)。结论补益强心片对慢性心力衰竭大鼠具有保护作用,其机制可能与激活PI3K/Akt信号通路、抑制凋亡有关。

Protective effects of Buyi Qiangxin Tablets on rats with chronic heart failure

AIM To study the protective effects of Buyi Qiangxin Tablets on rats with chronic heart failure.METHODS Sixty rats were randomly divided into normal control group, sham operation group, model group and low-dose, medium-dose, high-dose Buyi Qiangxin Tablets groups(0.18, 0.36, 0.72 g/kg), after which the rat models for chronic heart failure were established by ligating left anterior descending coronary artery. After 4-week intragastric administration, hemodynamic parameters(LVEDd, LVESd, LVEF) were detected by ultrasonic apparatus, BNP and ANP levels were detected by ELISA, cell apoptosis rate was detected by flow cytometry, Bcl-2, Bax and caspase-3 mRNA expressions were detected by RT-PCR, Bcl-2, Bax, caspase-3, PI3 K, p-PI3 K, Akt and p-Akt protein expressions were detected by Western blot.RESULTS Compared with the model group, the Buyi Qiangxin Tablets groups demonstrated significantly improved hemodynamic parameters(P<0.05, P<0.01), markedly decreased BNP and ANP levels, cell apoptosis rate, and Bax, caspase-3 mRNA and protein expressions(P<0.01), obviously increased Bcl-2 mRNA and protein expressions, p-PI3 K/PI3 K and p-Akt/Akt(P<0.05, P<0.01). CONCLUSION Buyi Qiangxin Tablets exhibit protective effects on rats with chronic heart failure, whose mechanisms may contribute to the activation of PI3 K/Akt signaling pathway and inhibition of apoptosis.