HPLC法同时测定淫羊藿-骨碎补药对中7种成分

目的建立HPLC法同时测定淫羊藿-骨碎补药对中朝藿定A、新北美圣草苷、柚皮苷、朝藿定B、朝藿定C、淫羊藿苷、宝藿苷Ⅰ的含有量。方法淫羊藿-骨碎补饮片60%乙醇提取物的分析采用采用Waters X Bridge C18柱(4.6 mm×250 mm,5μm);流动相乙腈-0.1%磷酸,梯度洗脱;柱温25℃;体积流量1.0 mL/min;检测波长280 nm。结果 7种成分在各自范围内线性关系良好(r≥0.9991);平均加样回收率95.06%~103.9%,RSD 1.1%~2.7%。结论该方法准确稳定,重复性好,可用于淫羊藿-骨碎补药对的质量控制。

Simultaneous determination of seven constituents in herbal pair of Epimedii Folium and Drynaria Rhizoma by HPLC

AIM To establish an HPLC method for the simultaneous content determination of epimedin A, neoeriocitrin, naringin, epimedin B, epimedin C, icariin and baohuoside Ⅰ in herbal pair of Epimedii Folium-Drynaria Rhizoma.METHODS The analysis of 60% ethanol extract of Epimedii Folium-Drynaria Rhizoma was performed on a 25 ℃ thermostatic Waters X Bridge C18 column(4.6 mm×250 mm, 5 μm), with the mobile phase comprising of acetonitrile-0.1% phosphoric acid flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelength was set at 280 nm.RESULTS Seven constituents showed good linear relationships within their own ranges(r ≥ 0.9991),whose average recoveries were 95.06%-103.9% with the RSDs of 1.1%-2.7%.CONCLUSION This accurate,stable and reproducible method can be used for the quality control of herbal pair,Epimedii Folium-Drynaria Rhizoma.