| 一种改进的大脑皮层神经元原代培养方法的研究 |
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| 引用本文： | 周国凤,汤京龙,周亮,奚廷斐.一种改进的大脑皮层神经元原代培养方法的研究[J].药物分析杂志,2011(2). |
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| 作者姓名： | 周国凤 汤京龙 周亮 奚廷斐 |
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| 作者单位： | 温州医学院;中国药品生物制品检定所;北京大学深圳研究院; |
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| 基金项目： | 国家自然科学基金(30770579); 973项目(2007C13936101); 863项目(2007AA021901); 中国药品生物制品检定所中青年研究发展基金(2009C4)资助 |
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| 摘 要： | 目的:建立1种简单、高效的新生乳鼠大脑皮层神经元原代培养的方法。方法:出生12 h以内的SD乳鼠为研究对象,采用胰酶消化和机械分离法相结合制备细胞悬液;在细胞培养过程中,不加入阿糖胞苷抑制神经胶质细胞生长,而是用2%B27 Neurobasal A medium维持培养。结果:神经元生长良好,形成神经元网络系统;通过神经元免疫组织化学鉴定神经元纯度达90%以上。结论:此方法是获得纯度较高的神经元的1种可靠方法。
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| 关 键 词： | 神经元 细胞培养 乳鼠 |
Research on an improved method for primary culture of cerebral cortex neurons |
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| ZHOU Guo-feng,TANG Jing-long,ZHOU Liang,XI Ting-fei.Research on an improved method for primary culture of cerebral cortex neurons[J].Chinese Journal of Pharmaceutical Analysis,2011(2). |
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| Authors: | ZHOU Guo-feng TANG Jing-long ZHOU Liang XI Ting-fei |
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| Affiliation: | ZHOU Guo-feng1,2,TANG Jing-long2,ZHOU Liang1,XI Ting-fei1,3(1.Wenzhou Medical College,Wenzhou 325035,China,2.National Institute for the Control of Pharmaceutical and Biological Products,Beijing 100050,3.Shenzhen Institute of Peking University,Shenzhen 510087,China) |
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| Abstract: | Objective:To establish an simple and effective primary culture skill suitable to neurons of newborn rat cortical tissure in vitro.Method:Objects of study were newborn SD rats which born within 12 h,combination trypsin digestion and mechanical dissociation were adopted to conduct culture;in the course of culture,arabinosid cytarabine(Arc-C) was not put to use to inhibit the outgrowth of glia cell,2%B27 Neurobasal A medium maintain cell growth.Result:The neurons grew well and formed nerver net system;purity o... |
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| Keywords: | neuron cell culture newborn rat |
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