| 乌头碱含量测定方法的建立 |
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| 引用本文： | 付宇,高建,赵宇,孙艳芳,刘秀梅,丁笑丽.乌头碱含量测定方法的建立[J].化学工程师,2010,24(11):29-30. |
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| 作者姓名： | 付宇 高建 赵宇 孙艳芳 刘秀梅 丁笑丽 |
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| 作者单位： | 黑龙江省疾病控制中心,黑龙江哈尔滨150030 |
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| 摘 要： | 目的:建立用高效液相色谱测定保健食品中功效成分乌头碱含量的方法。方法:样品中的乌头碱经浓氨试液-甲醇(2∶20)混合溶液加热回流,以乙醚振摇萃取,甲醇溶解并定容,经C18柱分离,在紫外检测器232nm波长处检测。结果:此方法的回收率为85.8%～92.7%,RSD为6.7%。结论:此方法操作简单,准确可靠,为保健食品中乌头碱含量的检测提供参考。
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| 关 键 词： | 乌头碱 高效液相液相色谱法 |
Establishment of cotent of aconitine determination method |
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| FU Yu,GAO Jian,ZHAO Yu,SUN Yan-fang,LIU Xiu-mei,DING Xiao-li.Establishment of cotent of aconitine determination method[J].Chemical Engineer,2010,24(11):29-30. |
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| Authors: | FU Yu GAO Jian ZHAO Yu SUN Yan-fang LIU Xiu-mei DING Xiao-li |
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| Affiliation: | (Heilongjiang Conter for Disease Control and Preuertion,Harbin 150030,China) |
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| Abstract: | A high performance liquid chromatography（HPLC） method to determine aconitine in health food was established Aconitine in the sample was circumfluenced with ammonia-methyl alcohol and extracted with aether,the C18 column was adopted to separate an detected at wavelength 232nm.The rate of recovery was 86.2%～93.2%,RSD was 6.7%.The method is accurate reliable.It could provide reference for quality control of aconitine in health food. |
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| Keywords: | aconitine high performance liquid chromatography |
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