活血消异方对子宫内膜异位症模型小鼠体外受精胚胎发育的影响

目的 评估活血消异方子宫内膜异位症模型小鼠体外受精胚胎发育的影响。方法 通过体外受精的方法, 观察中药组、模型组和假手术组的小鼠的促排卵数、受精率、二细胞分裂率、四细胞分裂率、桑葚胚形成率及最终桑葚胚形成率, 观察对子宫内膜异位症模型小鼠体外受精胚胎发育的影响。探讨内异症对小鼠体外受精后胚胎发育的影响及活血消异方对胚胎发育的作用。结果 中药组、模型组和假手术组, 共三组。三组的平均促排卵数、体外受精率和二细胞分裂率差异有显著性意义(P<0.05), 平均促排卵数、体外受精率和二细胞分裂率由高到低均依次为假手术组、中药组和模型组;三组的四细胞分裂率差异有显著性意义(P<0.05), 桑葚胚形成率差异无显著性差异(P>0.05), 桑葚胚最终形成率差异有显著性差异(P<0.05)。结论 子宫内膜异位症可降低模型小鼠促排卵数、体外受精率及卵裂率, 影响胚胎发育质量,活血消异方可改善胚胎发育质量。

Effects of huoxue xiaoyi fang on embryo development after IVF in endometriosis model mice

Objectives To evaluate the effect of a Chinese traditional medicine, Huoxue Xiaoyi Fang, on the embryo development after in vitro fertilization (IVF) in mouse models of endometriosis. Methods To observe the quantity of oocytes including the average number of superovulation, fertility rate, 2-cell and 4-cell embryo cleavage rates at different stages in vitro fertilization, and morulation rate among the traditional Chinese medicine group (TCM group), endometriosis model group (EM model group) and sham-operated group. To explore the influence of endometriosis and the effect of Huoxue Xiaoyi Fang on the quantity of oocytes and embryonic development after IVF in the mouse models of endometriosis. Results All the average number of superovulation, fertility rate, 2-cell and 4-cell embryo cleavage rates in the three groups showed significant differences (P<0.05), and the average number of superovulation was in an order of sham operated group>TCM group>model group. There were no significant differences in the morulation rates in the three groups (P>0.05). There were significant differences among the IVF rates of the three groups (P<0.05), and to be in an order of sham operated group>TCM group>model group. There were significant differences in the final morulation rate among the three groups (P<0.05). Conclusions Endometriosis may influence the quality of embryonic development of EM model mice, including reduced average number of superovulation, fertility rate, and embryo cleavage rate. The Chinese traditional medicine Huoxue Xiaoyi Fang can improve the the quantity of embryonic development after IVF.