Th1/Th2细胞因子在妊高征患者胎盘组织中的表达

目的 :探讨T辅助细胞 (Th) 1/Th2细胞因子在妊娠高血压综合征 (PIH)孕妇和血压正常的晚期妊娠妇女 (NLP)胎盘中的表达规律 ,从免疫学角度研究妊高征的发病原因及机制。方法 :选取PIH和NLP孕妇作为研究组和对照组 ,应用原位杂交法对两组胎盘的Th1型细胞因子 肿瘤坏死因子 (TNF)α、白细胞介素 (IL) 2 ]和Th2型细胞因子 (IL 10 )进行标记并通过彩色病理图像分析系统对染色结果进行定量检测并作比较。结果 :(1)TNFαmRNA、IL 2mRNA在PIH及NLP组胎盘合体滋养细胞表达的平均光密度分别为0 .1978± 0 .0 32 1、0 .2 0 39± 0 .0 4 11及 0 .16 79± 0 .0 30 9、0 .16 0 0± 0 .0 4 46 (P <0 .0 0 1) ,随着PIH病情加重 ,表达逐渐增强 (P <0 .0 5 ) ;(2 )IL 10mRNA在PIH及NLP组胎盘合体滋养细胞表达的平均光密度分别为 0 .15 6 4± 0 .0 4 36及 0 .2 0 17± 0 .0 32 1(P <0 .0 0 1) ,随着PIH病情加重 ,表达逐渐减弱 (P <0 .0 5 )。结论 :在妊高征孕妇胎盘中表现为免疫杀伤的Th1型细胞因子表达增强 ,与病情呈正相关。表现为免疫保护或免疫营养的Th2型细胞因子则表达减弱 ,与病情呈负相关。提示母胎界面的Th1/Th2细胞因子平衡偏离可能是导致PIH发病的病因之一。

The expressions of Th1/Th2 cytokines in the placentas of patients with pregnancy induced hypertension

Objective:To explore the immune pathogenesis of pregnancy induced hypertension (PIH),the expression of T helper cell (Th)1/Th2 cytokines on the human placentas from patients with PIH and women with normotensive late pregnancy (NLP) were investigated.Methods:32 cases of NLP women and 54 cases of PIH patients in the third trimester were observed.The expression of Th1 cytokines tumor necrosis factor alpha(TNFα),interleukin 2(IL 2)] messenger RNA(mRNA) and Th2 cytokines(IL 10) mRNA on the placentas were detected by in situ hybridization method.Furthermore,multi function precise color image measure system was used for quantitative analysis.Results:(1)The average optical densities of TNFα mRNA and IL 2 mRNA in PIH group (0.1978±0.0321 and 0.2039±0.0411) were significantly higher than those of NLP group (0.1679±0.0309 and 0.1600±0.0466) (P<0.001). The expression of TNFα mRNA and IL 2 mRNA in slight PIH was significantly lower than those in moderate and severe PIH (P<0.05);(2)The average optical densities of IL 10 mRNA in PIH group (0.1564±0.0436) were significantly lower than those of NLP group (0.2017±0.0321)(P<0.001). The expression of IL 10 mRNA in slight PIH was significantly higher than those in moderate and severe PIH (P<0.05).Conclusions:The expression levels of Th1 cytokines those mostly play an immunologic injure role in pregnancy in PIH group are higher than those in NLP group,but Th2 cytokines (IL 10 mRNA) those mostly play an immune protection or nutritious role in pregnancy are reversely.Furthermore,with the development of PIH,the expression levels of Th1 cytokines become higher but Th2 cytokines become lower.These results suggest that the Th1/Th2 cytokines imbalance in maternal fetal interface is one of the pathogenesis of PIH.