| 3,5-二硝基水杨酸与牛血清白蛋白相互作用的荧光光谱研究 |
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| 引用本文： | 王志军,谢晶,雷海英,梁瑞瑞.3,5-二硝基水杨酸与牛血清白蛋白相互作用的荧光光谱研究[J].山西大学学报(自然科学版),2012,35(1):85-88. |
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| 作者姓名： | 王志军 谢晶 雷海英 梁瑞瑞 |
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| 作者单位： | 1. 长治学院化学系,山西长治,046011
2. 长治学院生物科学与技术系,山西长治,046011 |
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| 基金项目： | 山西省高校科技开发项目(20081042);山西省高等学校大学生创新创业训练项目(2011362);长治学院校级资助项目(2011116) |
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| 摘 要： | 应用荧光光谱研究了3,5-二硝基水杨酸(DNS)与牛血清白蛋白(BSA)分子间的相互作用.研究表明:DNS对BSA内源荧光的猝灭机制属于形成复合物所引起的静态猝灭；结合位点数为1.同步荧光光谱显示DNS与BSA的结合位点更接近于色氨酸残基.根据F(o)rster非辐射能量转移理论,计算了授体-受体间的结合距离.
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| 关 键 词： | 3 5-二硝基水杨酸 牛血清白蛋白 荧光光谱 同步荧光 |
Spectra Study on the Interaction of 3,5-Dinitrosalicylic Acid with Bovine Albumin |
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| WANG Zhi-jun
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XIE Jing
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LEI Hai-ying
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LIANG Rui-rui.Spectra Study on the Interaction of 3,5-Dinitrosalicylic Acid with Bovine Albumin[J].Journal of Shanxi University (Natural Science Edition),2012,35(1):85-88. |
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| Authors: | WANG Zhi-jun XIE Jing LEI Hai-ying LIANG Rui-rui |
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| Affiliation: | 1( 1.Department of Chemistry,Changzhi University,Changzhi 046011,China; 2.Department of Biological sciences and Biotechnolog;Changzhi University,Changzhi 046011,China) |
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| Abstract: | The interaction between 3,5-dinitrosalicylic acid and bovine serum albumin were studied by fluorescence spectroscopy.The results shown that the quenching mechanism of the combination of bovine serum with 3,5-Dinitrosalicylic acid was a static quenching procedure.The number of binding sites was 1.The synchronous fluorescence indicated that the binding site of DNS with BSA was near by tryptophan subunit.The binding distance between 3,5-dinitrosalicylic acid with bovine serum albumin and the energy transfer efficiency were obtained based on the theory of Frster spectroscopy energy transfer. |
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| Keywords: | 3 5-Dinitrosalicylic acid bovine serum fluorescence spectroscopy synchronous fluorescence |
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