| Alk-SMase基因敲除小鼠的饲养繁殖及鉴定 |
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| 引用本文： | 陈 橼,吴金东,钱 俊,朱顺星,邵冰峰,张素青,何向锋,杨俐萍,张一心.Alk-SMase基因敲除小鼠的饲养繁殖及鉴定[J].南京医科大学学报,2015(2):183-187. |
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| 作者姓名： | 陈 橼 吴金东 钱 俊 朱顺星 邵冰峰 张素青 何向锋 杨俐萍 张一心 |
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| 作者单位： | 南通大学附属肿瘤医院,江苏 南通 226001;江苏 南通 226001;南通大学附属肿瘤医院,江苏 南通 226001;江苏 南通 226001;南通大学研究生部,江苏 南通 226001;南通大学实验动物中心,江苏 南通 226001;南通大学附属肿瘤医院,江苏 南通 226001;江苏 南通 226001;南通大学附属肿瘤医院,江苏 南通 226001;江苏 南通 226001;南通大学附属肿瘤医院,江苏 南通 226001;江苏 南通 226001;南通大学附属肿瘤医院,江苏 南通 226001;江苏 南通 226001;南通大学附属肿瘤医院,江苏 南通 226001;江苏 南通 226001 |
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| 摘 要： | 目的 :为了繁育和鉴定碱性鞘磷脂酶(Alkaline sphingomyelinase,alk-SMase)基因敲除小鼠,将引进的基因敲除小鼠进行饲养繁殖,杂合子、纯合子用于继续保种。方法:对其幼鼠剪尾提取基因组DNA,采用PCR方法进行基因型鉴定;取alkSMase表达组织做HE染色进行形态学比较。结果 :对引进小鼠已成功饲养和繁殖,并得到纯合alk-SMase基因缺失型小鼠。结论:正确的饲养、繁殖及基因鉴定方法对于基因敲除小鼠的获得和保种具有重要的意义。
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| 关 键 词： | alk-SMase 基因敲除小鼠 PCR |
| 收稿时间： | 2014/3/26 0:00:00 |
Breeding and identification of Alk-SMase knockout mice |
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| Chen Yuan,Wu Jindong,Qian Jun,Zhu Shunxing,Shao Bingfeng,Zhang Suqing,He Xiangfeng,Yang Liping and Zhang Yixin.Breeding and identification of Alk-SMase knockout mice[J].Acta Universitatis Medicinalis Nanjing,2015(2):183-187. |
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| Authors: | Chen Yuan Wu Jindong Qian Jun Zhu Shunxing Shao Bingfeng Zhang Suqing He Xiangfeng Yang Liping and Zhang Yixin |
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| Affiliation: | The Affiliated Tumor Hospital of Nantong University,Nantong 226001;The Affiliated Tumor Hospital of Nantong University,Nantong 226001;Department of Postgraduate; Nantong University,Nantong 226001,China;Laboratory Animal Center Nantong University,Nantong 226001,China;The Affiliated Tumor Hospital of Nantong University,Nantong 226001;The Affiliated Tumor Hospital of Nantong University,Nantong 226001;The Affiliated Tumor Hospital of Nantong University,Nantong 226001;The Affiliated Tumor Hospital of Nantong University,Nantong 226001;The Affiliated Tumor Hospital of Nantong University,Nantong 226001 |
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| Abstract: | Objective:To breed and identify of alkaline sphingomyelinase(Alkaline sphingomyelinase,alk-SMase)knockout mice. Methods:DNA was extracted from the mice tissue. PCR method was used for genotype identification. HE staining was used for morphology identification. Results:The gene kwockout mice has been successfully feed and breeded,and the generated alk-SMase knockout (KO) mice were gotten. Conclusion:The correct way to feed,breed and gene identification for gene knockout mice has important significance for obtainmont and transgenic mice preservation. |
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| Keywords: | alk-SMase knockout mice PCR |
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