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DNA Extraction from Eriocaulon Plants and Construction of RAPD System
作者姓名:XueXian  LinShanzhi  ZhangZhixiang
作者单位:KeyLaboratoryforGeneticsandBreedinginForestTreesandOrnamentalPlants,MOE,BeijingForestryUniversity,Beijing100083,P.R.China
基金项目:教育部科学技术研究项目
摘    要:There have been many arguments on the classification of Eriocaulon Linn. by morphology so far, and little is known about the use of molecular marker for genetic for genetic diversity of Eriocaulon plants. To apply the technique of molecular marker to the research of genetic diversity of Eriocaulon plants, the study of the extraction method of DNA from the Eriocaulon plants and the RAPD system are essential for researchers. In this paper, the extraction of genome DNA from the silica-gel-dried leaves of several species of Eriocaulon distributed in China was studied, and the best RAPD analysis technique condition of Eriocaulon plants was analyzed.

关 键 词:DNA  脱氧核糖核酸  提取  RAPD  植物  基因转染
收稿时间:2003-6-5

DNA Extraction from Eriocaulon Plants and Construction of RAPD System
XueXian LinShanzhi ZhangZhixiang.DNA Extraction from Eriocaulon Plants and Construction of RAPD System[J].Forestry Studies in China,2004,6(1):22-26.
Authors:XUE Xian  Lin Shanzhi  Zhang Zhixiang
Affiliation:Key Laboratory for Genetics and Breeding in Forest Trees and Ornamental Plants, MOE, Beijing Forestry University, Beijing 100083, P. R. China
Abstract:There have been many arguments on the classification of Eriocaulon Linn. by morphology so far, and little is known about the use of molecular marker for genetic for genetic diversity of Eriocaulon plants. To apply the technique of molecular marker to the research of genetic diversity of Eriocaulon plants, the study of the extraction method of DNA from the Eriocaulon plants and the RAPD system are essential for researchers. In this paper, the extraction of genome DNA from the silica-gel-dried leaves of several species of Eriocaulon distributed in China was studied, and the best RAPD analysis technique condition of Eriocaulon plants was analyzed.
Keywords:Eriocaulon  DNA extraction  RAPD  genetic diversity  
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