| 缓冲体系对产弹性蛋白酶芽孢杆菌EL31410发酵的影响 |
| |
| 引用本文： | 徐莹,何国庆,陈启和,李景军.缓冲体系对产弹性蛋白酶芽孢杆菌EL31410发酵的影响[J].农业生物技术学报,2004,12(6):709-713. |
| |
| 作者姓名： | 徐莹 何国庆 陈启和 李景军 |
| |
| 作者单位： | 1. 浙江大学食品科学与营养系,杭州,310029
2. 江南大学食品学院,无锡,214036 |
| |
| 摘 要： | 摘要: 弹性蛋白酶是以降解弹性硬蛋白为特征的蛋白水解酶。培养芽孢杆菌(Bacillus sp.) EL31410产弹性蛋白酶的过程中，pH的波动对产酶有较大影响。菌体最适生长pH为6.0～7.0，最适产酶pH为6.4～6.6。采用单因素实验和二次通用旋转回归设计研究了缓冲体系对产弹性蛋白酶芽孢杆菌EL31410发酵的影响。优化结果显示：15 h后添加pH 6.4 KH2PO4-NaOH缓冲液，使其在培养基中浓度为0.02 mol/L时，对控制发酵液pH和提高菌体生长及酶活性的综合效果为最佳。验证实验证明该模型是较可靠的。
|
| 关 键 词： | 关键词: Bacillus sp.EL31410 弹性蛋白酶 缓冲液 pH |
| 修稿时间： | 2003年12月29 |
Effect of Buffer Systems on Elastase Production by Bacillus sp.EL31410 |
| |
| XU Ying,HE Guo-qing,CHEN Qi-He,LI Jing-Jun.Effect of Buffer Systems on Elastase Production by Bacillus sp.EL31410[J].Journal of Agricultural Biotechnology,2004,12(6):709-713. |
| |
| Authors: | XU Ying HE Guo-qing CHEN Qi-He LI Jing-Jun |
| |
| Abstract: | Abstract：The elastase is a kind of protease that can hydrolyse elastin. pH is one of the most important environmental factors for cell growth and product accumulation. The best pH for cell growth and elastase production were about 6.0～7.0 and 6.4～6.6, respectively. Single factor and rotatable orthogonal central composite design were used to study the effect of buffer systems on producing elastase. The optimal result showed that 15 h later adding pH 6.4 (0.02 mol/L KH2PO4-NaOH) buffer to medium was the best way for cell growth and elastase production. This model was verified to be credible. |
| |
| Keywords: | |
| 本文献已被 维普 万方数据 等数据库收录！ |
| 点击此处可从《农业生物技术学报》浏览原始摘要信息 |
