| 超低温冷冻对羯布罗香种子结构和生理生化特性的影响 |
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| 引用本文： | 曾琳,顾雅坤,吴怡,郑希龙.超低温冷冻对羯布罗香种子结构和生理生化特性的影响[J].热带亚热带植物学报,2018,26(3):249-254. |
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| 作者姓名： | 曾琳 顾雅坤 吴怡 郑希龙 |
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| 作者单位： | 中国医学科学院药用植物研究所海南分所 |
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| 基金项目： | 国家青年科学基金项目（81603399）；中央高校基本科研业务费专项（协和青年基金）（3332016071）；中国医学科学院医学与健康科技创新工程项目（2016-I2M-2-003）资助 |
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| 摘 要： | 为长期、稳定、有效地保存羯布罗香(Dipterocarpus turbinatus Gaertn.F.)种子,探讨了低温冷藏对种子活力、结构和生理生化指标的影响。结果表明,45%的含水量是种子的安全储藏含水量;玻璃化冷冻和缓慢冷冻对种子结构损害较大,且冷冻后的种子活力下降幅度大;快速冷冻法是种子超低温保存的最佳冷冻方式;液氮冷冻降低了种子过氧化物酶、过氧化氢酶和脱氢酶活性,提高了α-淀粉酶活性,而对丙二醛含量无显著影响。因此,超低温保存羯布罗香种子是科学可行的。
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| 关 键 词： | 超低温冷冻 羯布罗香 种子 结构 生理生化 |
| 收稿时间： | 2017/9/18 0:00:00 |
| 修稿时间： | 2017/11/15 0:00:00 |
Effect of Cryopreservation on Structure, Physiology and Biochemical Characteristics of Dipterocarpus turbinatus Seeds |
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| ZENG Lin,GU Ya-kun,WU Yi and ZHENG Xi-long.Effect of Cryopreservation on Structure, Physiology and Biochemical Characteristics of Dipterocarpus turbinatus Seeds[J].Journal of Tropical and Subtropical Botany,2018,26(3):249-254. |
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| Authors: | ZENG Lin GU Ya-kun WU Yi and ZHENG Xi-long |
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| Affiliation: | Hainan Branch Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Haikou 570311, China,Hainan Branch Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Haikou 570311, China,Hainan Branch Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Haikou 570311, China and Hainan Branch Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Haikou 570311, China |
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| Abstract: | |
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| Keywords: | Cryopreservation Dipterocarpus turbinatus Seed Structure Physiological and biochemical |
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