| 一组抗肺癌单克隆抗体的产生及其鉴定 |
| |
| 引用本文： | 刘亮,曹双,侯健存.一组抗肺癌单克隆抗体的产生及其鉴定[J].中国医学科学院学报,1988(5). |
| |
| 作者姓名： | 刘亮 曹双 侯健存 |
| |
| 作者单位： | 中国医学科学院基础医学研究所 北京
(刘亮，曹双)，中国医学科学院基础医学研究所 北京(侯健存) |
| |
| 摘 要： | 以肺癌细胞株和组织为免疫原制备4株抗肺癌McAbs,其中LC86a H8和LC86bD7可分别识别肺鳞癌不同亚类抗原,LC86a C5可识别肺腺癌某一亚类抗原,LC86a E4可识别某些肺鳞癌和肺腺癌的共同抗原。将这4株McAbs组成一组使用,可提高肺癌的检出率。
|
| 关 键 词： | 肺癌 单克隆抗体 |
Generation and Identify of a Panel of Monoclonal Antibodies against Carcinoma of Lung |
| |
| Liu Liang,et al.Generation and Identify of a Panel of Monoclonal Antibodies against Carcinoma of Lung[J].Acta Academiae Medicinae Sinicae,1988(5). |
| |
| Authors: | Liu Liang |
| |
| Abstract: | Monoclonal antibodies (McAb) were generated by fusing murine myeloma SP2/0 celts with spleen cells from BALB/c mouse immunized with lung adenocarcinoma cell line LTEP-a-2 and extracts of human lung squamous cell carcinoma obtained from surgical operation. The hybrids were screened for antibody specificity with different cell lines and fresh cryostat sections by indirect immunofluorescence assay. Four McAbs designated LC86a H8, LC86b D7, LC86a C5 and LC86a E4 were identified from 25 hybrids. It was found that LC86a H8 only reacted with lung poorly differentiated squamous cell carcinomas; LC86b D7 bound only to lung highly differentiated squamous cell carcinomas; LC86a C5 was only reactive with a part of lung adenocarcinomas and LC86a E4 could bind to some of lung squmaous cell carcinomas and lung adenocarcinomas. Besides, lung cancer antigens were found existing in supernatant culture fluid of lung adenocarcinoma cells. These McAbs capable of recognizing different subtypes of lung cancers formed a group to be used for promoting early diagnosis of carcinomas of lung. |
| |
| Keywords: | lung cancer monoclonal antibodies |
| 本文献已被 CNKI 等数据库收录！ |
|
