| 诱导时机对羧肽酶原B包涵体质量和复性率影响的研究 |
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| 引用本文： | 辛爱洁,王亚辉,李素霞,袁勤生.诱导时机对羧肽酶原B包涵体质量和复性率影响的研究[J].食品与药品,2009(7). |
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| 作者姓名： | 辛爱洁 王亚辉 李素霞 袁勤生 |
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| 作者单位： | 华东理工大学生物反应器工程国家重点实验室; |
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| 摘 要： | 目的研究诱导时机对羧肽酶原B包涵体质量和复性率的影响,探讨包涵体质量评价体系。方法在不同时间诱导产生重组羧肽酶原B包涵体,研究其变性和复性、对蛋白酶K的稳定性及在一定浓度盐酸胍溶液中的化学变性,分析诱导时间与包涵体复性率的相关性。结果诱导时机不同,所得重组羧肽酶原B包涵体复性率及对蛋白酶K的稳定性不同,在一定浓度盐酸胍溶液中的溶解性也不同。复性率越高的包涵体越不易被蛋白酶K酶解,对化学变性剂的稳定性也高。结论诱导时机影响重组羧肽酶原B包涵体的质量及复性率。
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| 关 键 词： | 羧肽酶B 诱导时机 包涵体 蛋白酶K |
Effect of Induction Time on Quality and Renaturation of Inclusion Bodies with Procarboxypeptidase B |
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| XIN Ai-jie,WANG Ya-hui,LI Su-xia,YUAN Qin-sheng.Effect of Induction Time on Quality and Renaturation of Inclusion Bodies with Procarboxypeptidase B[J].Food and Drug,2009(7). |
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| Authors: | XIN Ai-jie WANG Ya-hui LI Su-xia YUAN Qin-sheng |
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| Affiliation: | State Key Laboratory of bioreactor Engineering;East China University of Science and Technology;Shanghai 200237;China |
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| Abstract: | Objective To study the effect of induction time on the quality and renaturation of inclusion bodies with procarboxypeptidase B and approach the evaluation system on the inclusion bodies.Methods The inclusion bodies with recombinant procarboxypeptidase B(pCPB IBs) were obtained by induction at different time.The denaturation and renaturation of pCPB IBs,the stability against protease K and chemical denaturation in GuHCl solutions with certain concentrations were studied.The correlation between induction time... |
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| Keywords: | carboxypeptidase B induction time inclusion body protease K |
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