| HPLC法测定地萸丸中盐酸小檗碱的含量 |
| |
| 引用本文： | 曹毅,陈静.HPLC法测定地萸丸中盐酸小檗碱的含量[J].齐鲁药事,2011,30(9):517-518. |
| |
| 作者姓名： | 曹毅 陈静 |
| |
| 作者单位： | 1. 淄博市张店区中医院,山东,淄博,255000
2. 淄博市药品检验所,山东,淄博,255000 |
| |
| 摘 要： | 目的建立高效液相色谱法测定地萸丸中盐酸小檗碱含量的方法.方法色谱柱为InertsilODS-SP(4.6 mm×150mm,5μm);流动相为乙腈-0.033 mol·L-1磷酸二氢钾溶液(30∶70);检测波长为345 nm;流速为1.0 mL·min-1.结果盐酸小檗碱在4.172～41.72μg·mL-1(r=0.999 9)线性关系良好,平均回收率为98.7%,RSD=0.80%.结论本法简便、快速、重复性好,可用于地萸丸的质量控制.
|
| 关 键 词： | 地萸丸 盐酸小檗碱 含量测定 高效液相色谱法 |
Determination of berberine hydrochloride in Diyu Pills by HPLC |
| |
| CAO Yi,CHEN Jing.Determination of berberine hydrochloride in Diyu Pills by HPLC[J].qilu pharmaceutical affairs,2011,30(9):517-518. |
| |
| Authors: | CAO Yi CHEN Jing |
| |
| Affiliation: | CAO Yi1,CHEN Jing2(1.Zhangdian Traditional Chinese Medical Hospital of Zibo,Zibo 255000,China,2.Zibo Institute forDrug Control,China) |
| |
| Abstract: | Objective To establish an HPLC method for the determination of berberine hydrochloride in Diyu Pills.Methods Berberine hydrochloride was separated on Inertsil ODS-SP（4.6 mm×150 mm,5 μm）column.The mobile phase was acetonitrile-0.033 mol·L-1 potassium dihydrogen phosphate solution（30∶ 70）.The detection wavelength was 354 nm.The flow rate was 1.0 mL·min-1.Resules The linear range of berberine hydrochloride was 4.172～41.72 μg·mL-1（r=0.999 9）.The average recovery was 98.7% with RSD=0.80%.Conclusion The method was simple,rapid reproducible,and it can be used for the quality control of Diyu Pills. |
| |
| Keywords: | Diyu Pills Berberine hydrochloride Determination HPLC |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
