| 杜仲组织培养的研究进展 |
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| 引用本文： | 唐亮,金晓玲.杜仲组织培养的研究进展[J].贵州农业科学,2010,38(3). |
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| 作者姓名： | 唐亮 金晓玲 |
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| 作者单位： | 中南林业科技大学,环境艺术设计学院,湖南,长沙,410004 |
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| 基金项目： | 国家“十一五”科技支撑计划子专题“杜仲优良无性系快速微繁殖技术研究”(2006BAD18B0303) |
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| 摘 要： | 为了解杜仲组织培养的研究现状,对杜仲组织培养的研究进行了综述。杜仲是通过器官发生途径获得再生植株的,所用的外植体有叶片、叶柄、腋芽、胚轴、带芽茎段、茎尖和子叶等7种,除子叶外都成功组培获得了小植株。基本培养基、激素和附加营养成分等是影响组织培养的重要因素,其中,基本培养基以MS使用最多,初代培养中占83.3%,继代培养也有66.7%,而66.7%的生根培养基采用的是1/2MS;芽的诱导中使用的激素有BA、NAA、IBA、BAP、IAA、KT、TDZ等7种,其中MS+0.1mg/L6-BA效果最好,诱导率达到96.7%;BA、NAA、IAA、BAP、GA3等5种激素对芽的增殖起作用,其中以MS+2.0mg/LBA+0.05mg/LNAA效果最好,增殖系数达3.69;而生根所需激素主要有NAA、IBA、GA,其中以IBA1.5mg/L或2.0mg/L效果最好,生根率达到92.5%;杜仲组织培养中常用的附加营养成分有酵母浸提物和谷氨酰胺等。
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| 关 键 词： | 杜仲 组织培养 器官发生 植株再生 |
Advance in Tissue Culture of Eucommia ulmoides |
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| TANG Liang,JIN Xiao-ling.Advance in Tissue Culture of Eucommia ulmoides[J].Guizhou Agricultural Sciences,2010,38(3). |
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| Authors: | TANG Liang JIN Xiao-ling |
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| Affiliation: | School of Environmental Art & Desigh;Central South University of Forestry Technology;Changsha;Hunan 410004;China |
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| Abstract: | The paper reviewed advance in tissue culture of Eucommia ulmoides to provide a reference for its further research. The plantlets of Eucommia ulmoides can be obtained by the organogenesis path from its leaf,petiole,axillary bud,plumular axis,stem section with a bud and stem apex successfully. The used basic medium for its primary culture and subculture is MS,and 1/2 MS is used in its rooting culture. The used hormones for its bud induction mainly are BA,NAA,IBA,BAP,IAA,KT and TDZ and 96.7% bud induction rate... |
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| Keywords: | Eucommia ulmoides tissure culture organogenesis plantlet regeneration |
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