Effect of arctigenin on apoptosis of human nasopharyngeal carcinoma cell line CNE-1 |
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Authors: | HUANG Dong-dong MENG Yi-yu SUN Dong-xun JIN Qiao-zhi CHEN Wu-bing CAI Zhi-yi |
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Affiliation: | 1. The First Clinical Medical Institute, Wenzhou Medical University, Wenzhou 325000, China;
2. Department of Otolaryngology, Taizhou Municipal Hospital, Taizhou 318000, China |
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Abstract: | AIM: To investigate the effect of arctigenin on the apoptosis of human nasopharyngeal carcinoma cell line CNE-1 and its potential mechanism. METHODS: The inhibition of cell viability was analyzed by CCK-8 assay. The activity of caspase-3 and caspase-9 was analyzed by caspase-3 and caspase-9 activity kit. Apoptotic cell percentage was evaluated by Annexin V-PI staining. The expression of PI3K/AKT/XIAP signal pathway-related molecules at mRNA and protein levels was analyzed by real-time PCR and Western blot. RESULTS: Arctigenin inhibited the cell activity in a dose- and time-dependent manner after treatment with arctigenin at concentrations of 10, 20, 40 and 80 μmol/L for 24 h, 48 h and 72 h (P<0.01). Arctigenin also increased the activity of caspase-3 and caspase-9 and the apoptotic rate (P<0.05), and down-regulated the mRNA and protein expression of PI3K/AKT/XIAP signal pathway-related molecules (P<0.05).CONCLUSION: Arctigenin induces the apoptosis of CNE-1 cells through PI3K/AKT/XIAP signal pathway. |
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Keywords: | Arctigenin Apoptosis Nasopharyngeal carcinoma |
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