卵巢浆液性囊腺癌组织中高尔基磷酸化蛋白3的表达及其意义

目的 研究高尔基磷酸化蛋白3（GOLPH3）在卵巢浆液性囊腺癌组织中的表达及意义.方法 应用实时荧光定量PCR、Western blot分别检测正常卵巢组织、卵巢浆液性囊腺瘤、卵巢浆液性囊腺癌组织中GOLPH3在mRNA和蛋白水平的表达.结果 GOLPH3 mRNA在卵巢浆液性囊腺瘤和浆液性囊腺癌组织中均有表达,卵巢浆液性囊腺癌组织中表达高于良性肿瘤组（2.97～7.04倍）,差异有统计学意义（P＜0.05）.GOLPH3蛋白在正常卵巢组织中未检测到表达,卵巢浆液性囊腺癌组织中阳性表达率73.8％,明显高于良性肿瘤组的35.71％（P＜0.01）.GOLPH3蛋白阳性表达率与浆液性囊腺癌的临床分期（P=0.019）、病理分级（P=0.042）及有无淋巴结转移有关（P=0.000）,与患者年龄无关（P＞0.05）;且肿瘤分化越低GOLPH3蛋白表达量越高（P＜0.01）.结论 GOLPH3的过表达在卵巢浆液性囊腺癌的发生、发展中意义重大,可能是卵巢癌早期诊断和临床治疗的潜在靶点.

Expression and clinicopathologic significance of Golgi phosphoprotein 3 in ovarian serous cystadenocarcinoma

Objective To investigate the expression of Golgi phosphoprotein 3 （GOLPH3） at protein and mRNA levels in serous epitheial ovarian carcinoma and its significance.Methods The expression of GOLPH3 at protein and mRNA levels were evaluaed by Western blot and Real time RT-PCR,respectively,in 42 cases of serous epithelium ovarian carcinoma,14 cases of benign serous ovarian tumors and 7 cases of normal ovarian epithelium tissues.Results GOLPH3 mRNA was significantly higher from 2.97 to 7.04 fold in ovarian serous cystadenocarcinoma tissues compared with ovarian serous carcinoma tissues （P ＜ 0.05）.There was no positive expression of GOLPH3 protein in normal ovarian epithelium tissues.GOLPH3 protein positive expression rate was higher in ovarian serous cystadenocarcinoma （73.81 ％） than that in ovarian serous carcinoma （35.71％） （P ＜ 0.01）.GOLPH3 protein was correlated with the pathologic differentiation （P =0.019）,the FIGO stage （P =0.042）,and lymphatic metastasis （P =0.000）,but was not associated with age （P =0,881）.The positive rate and overexpression of GOLPH3 in poor differentiated grade group were higher than that in well and middle differentiated grade group （P ＜ 0.05）.Conclusions GOLPH3 may play an important role on the development of ovarian serous cystadenocarcinoma,and may be a potential target of gene therapy.