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T-REX: software for the processing and analysis of T-RFLP data
Authors:Steven W Culman  Robert Bukowski  Hugh G Gauch  Hinsby Cadillo-Quiroz and Daniel H Buckley
Affiliation:(1) Department of Crop and Soil Sciences, Cornell University, 515 Bradfield Hall, Ithaca, NY, USA;(2) Computational Biology Service Unit, Cornell University, 620 Rhodes Hall, Ithaca, NY, USA;(3) Department of Crop and Soil Sciences, Cornell University, 519 Bradfield Hall, Ithaca, NY, USA;(4) Department of Microbiology, University of Illinois at Urbana-Champaign, Urbana, IL, USA;(5) Department of Crop and Soil Sciences, Cornell University, 705 Bradfield Hall, Ithaca, NY, USA;(6) Department of Land, Air and Water Resources, One Shields Avenue, University of California, 3150 Plant and Environmental Science, Davis, CA 95616, USA
Abstract:

Background  

Despite increasing popularity and improvements in terminal restriction fragment length polymorphism (T-RFLP) and other microbial community fingerprinting techniques, there are still numerous obstacles that hamper the analysis of these datasets. Many steps are required to process raw data into a format ready for analysis and interpretation. These steps can be time-intensive, error-prone, and can introduce unwanted variability into the analysis. Accordingly, we developed T-REX, free, online software for the processing and analysis of T-RFLP data.
Keywords:
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