Enhancement of immune response of HBsAg loaded poly (L-lactic acid) microspheres against Hepatitis B through incorporation of alum and chitosan |
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| Authors: | Sreenivas Pandit Erdal Cevher Mohammed Gulrez Zariwala Satyanarayana Somavarapu Professor H Oya Alpar |
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| Affiliation: | 1. Centre for Drug Delivery Research, School of Pharmacy, University of London, London, UK;2. Department of Pharmaceutical Technology, Faculty of Pharmacy, Istanbul University, Istanbul, Turkey |
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| Abstract: | Purpose: Poly (L-lactic acid) (PLA) microparticles encapsulating Hepatitis B surface antigen (HBsAg) with alum and chitosan were investigated for their potential as a vaccine delivery system.Methods: The microparticles, prepared using a water-in-oil-in-water (w/o/w) double emulsion solvent evaporation method with polyvinyl alcohol (PVA) or chitosan as the external phase stabilising agent showed a significant increase in the encapsulation efficiency of the antigen.Results: PLA-Alum and PLA-chitosan microparticles induced HBsAg serum specific IgG antibody responses significantly higher than PLA only microparticles and free antigen following subcutaneous administration. Chitosan not only imparted a positive charge to the surface of the microparticles but was also able to increase the serum specific IgG antibody responses significantly.Conclusions: The cytokine assays showed that the serum IgG antibody response induced is different according to the formulation, indicated by the differential levels of interleukin 4 (IL-4), interleukin 6 (IL-6) and interferon gamma (IFN-γ). The microparticles eliciting the highest IgG antibody response did not necessarily elicit the highest levels of the cytokines IL-4, IL-6 and IFN-γ. |
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| Keywords: | Hepatitis B surface antigen vaccine microsphere L-lactic acid)" target="_blank">poly (L-lactic acid) chitosan alum cytokine |
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