Surfactant-associated protein inhibits phospholipid secretion from type II cells |
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Authors: | Rice W R; Ross G F; Singleton F M; Dingle S; Whitsett J A |
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Affiliation: | Pediatrics/Neonatology Division, University of Cincinnati College of Medicine, Ohio 45267. |
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Abstract: | Secretion of 3H]phosphatidylcholine (3H]PC) from isolated rat pulmonary type II epithelial cells was inhibited by the surfactant-associated protein of Mr = 35,000 (SAP-35) purified from canine lung surfactant. SAP-35 inhibited 3H]PC secretion in a dose-dependent manner and significantly inhibited basal, phorbol ester, beta-adrenergic, and P2-purinergic agonist-induced 3H]PC secretion. SAP-35 significantly inhibited 3H]PC secretion from 1 to 3 h after treatment. The IC50 for inhibition of 3H]PC secretion by canine SAP-35 was 1-5 X 10(-6) g/ml and was similar for inhibition of both basal and secretagogue-stimulated release. Heat denaturation of SAP-35, addition of monoclonal anti-SAP-35 antibody, reduction and alkylation of SAP-35, or association of SAP-35 with phospholipid vesicles reversed the inhibitory effect on secretagogue-induced secretion. Inhibitory effects of SAP-35 were observed 3 h after cells were washed with buffer that did not contain SAP-35. Although SAP-35 enhanced reassociation of surfactant phospholipid with isolated type II cells, its inhibitory effect on secretion of 3H]PC did not result from stimulation of reuptake of secreted 3H]PC by type II cells. The inhibition of phospholipid secretion by SAP-35 was also not due to inhibition of PC or disaturated PC synthesis by SAP-35. SAP-35, the major phospholipid-associated protein in pulmonary surfactant, is a potent inhibitor of surfactant secretion from type II cells in vitro and may play an important role in homeostasis of surfactant in the alveolar space. |
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