| 谷氨酸毒性损伤致PC12细胞NMDAR与CB表达的变化 |
| |
| 引用本文： | 吴南,冯华,朱刚,王宪荣.谷氨酸毒性损伤致PC12细胞NMDAR与CB表达的变化[J].重庆医学,2003,32(2):137-139. |
| |
| 作者姓名： | 吴南 冯华 朱刚 王宪荣 |
| |
| 作者单位： | 第三军医大学西南医院脑外科,重庆,400038 |
| |
| 摘 要： | 目的 探讨谷氨酸毒性损伤后PC12细胞NMDAR与CB表达的变化及意义。方法 建立谷免氨酸毒性损伤模型后，应用免疫组化及流式细胞仪的方法，定位、定量观测PC12细胞NMDAR与CB表达的变化。结果 NMDAR表达于胞膜上，谷氨酸致其表达增强，CB表达于胞浆中，谷氨酸致其表达减弱，在4h时相较1h略有恢复。结论 NMDAR与CB在神经细胞继发性损伤中可能起着重要作用。
|
| 关 键 词： | 谷氨酸毒性损伤 PC12细胞 NMDAR CB NMDA受体 |
| 文章编号： | 1671-8348(2003)02-0137-03 |
The change of NMDAR and CB expression in PC12 cells after glutmate intoxication |
| |
| WU Nan,FENG Hua,ZHU Gang,et al..The change of NMDAR and CB expression in PC12 cells after glutmate intoxication[J].Chongqing Medical Journal,2003,32(2):137-139. |
| |
| Authors: | WU Nan FENG Hua ZHU Gang |
| |
| Abstract: | Objective To explore the expression of NMDAR and CB in PC12 cells after glutmate intoxication. Methods The change of NMDAR and CB expression in PC12 cells was quantitativelly and locationally observed with flow cytometry and immunehistochemistry after the establishment of cell Glutmate intoxication models. Results NMDAR receptors were expressed on cells membrane. The expression of NMDAR in PC12 cells was high after Glutmate intoxication; CB were expressed in cyto plasma in PC12 cells. The expression of CB was low after Glutmate intoxication.Conclusion NMDAR and CB might be playing an important role in secondary injury of neurons. |
| |
| Keywords: | glutmate PC12 cells NMDAR calbindin D28K(CB) |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
