| H2O2诱导的肝星状细胞凋亡后胶原的变化 |
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| 引用本文： | 洪峰,王爱民,杨晓明,耿焱,张莉.H2O2诱导的肝星状细胞凋亡后胶原的变化[J].中华实验外科杂志,2005,26(1):902-903. |
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| 作者姓名： | 洪峰 王爱民 杨晓明 耿焱 张莉 |
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| 作者单位： | 解放军第252医院消化科,河北保定,071000;军事医学科学院放射医学研究所; |
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| 摘 要： | Objective To explore the changes of collagen (COL) Ⅰ and COLⅢ after apoptosis of HSC induced by H2O2. Methods Different apoptosis rate of HSC-T6 induced by H2O2 ( 100 nmol/L) was evaluated by flow cytometry. RT-PCR was used to detect the gene expression levels of COL Ⅰ and COL Ⅲ. Results Apoptosis of HSC was induced by 100 nmol/L H2O2 with the poptosis rate being 5.86%, 58.55% ,and 71.98% ,respectively. The COL Ⅰ and COLⅢ were highly expressed in activated HSC,and decreased sharply as apoptosis increased. The changes in gene expression of COL Ⅰ were much more obvi-ous than those of COL Ⅲ. Conclusion Inducing apoptosis of HSC may decrease the gene expression of collagens.
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| 关 键 词： | 肝星状细胞 脱噬作用 胶原 基因表达 |
Changes of collagens after apoptosis of HSC induced by H2O2 |
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| Abstract: | |
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| Keywords: | Hepatic stellate cellsApoptosisCollagenGene expression |
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