甘蓝型油菜新不育系恢复材料花药培养影响因素的研究

本试验用油菜新不育胞质恢复系为材料进行花药培养,对花药培养的一些影响因素进行了初步研究,结果表明:①NER愈伤诱导以MS基本培养基较好,附加激素为:2,4-D 2mg/L、6-BA0.5mg/L、NAA 0.5mg/L最佳。②株龄不同愈伤诱导率差异显著。从110 d到141d,出愈率随着株龄增加而增加,差异达到0.01显著水平。到141d达到最高,后逐渐下降。③适当增加2,4-D浓度,2,4-D与6-BA混用有利于愈伤的诱导。④将6-BA浓度从 mg/L提高到4mg/L新不育系恢复材料绿苗诱导率增加,在2mg/L6-BA的激素水平上添加玉米素(ZT)0.5mg/L绿苗诱导率增加2.47%。

Influencing factors on anther culture of restorer of new cytoplasmic male sterile(NER)(Brassica napus L.)

Thirty varieties of NER(the restorers of new cytoplasmic male Sterile)(Brassica napus)were grown under uncontrolled temperature and light conditions,and their anthers were used as explants in vitro culture.The influencing factors of anther culture were investigated and the main results are as follow:(i)MS was the best suitable for callus induction of anther culture of NER.And high numbers of callus were induced by culturing the anthers on MS medium addition with 2,4-D mg/L,6-BA 0.5 mg/L NAA 0.5 mg/L.(ii) The donor plant age had obvious effect on the rate of callus induction.The difference was significant on 0.01level.Rate of callus induction was increased according the increase of the donor plant age from 110 days to 141 days,and the rate of callus induction was the highest 141dags,so descend according the increase of the donor plant age.(iii)The callus induction was improved by supplemented with 2,4-D or 2,4-D and 6-BA used together.(iV)The rate of green plant induction was increased 2.47 % enriched with 0.5 mg/L ZT significantly based on the level of 2 mg/l 6-BA,and addition of 6-BA from 2 mg/L to 4mg/L was suitable for the green plant induction.