| HPLC外标法测定左炔诺孕酮炔雌醚片含量 |
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| 引用本文： | 简淑娟.HPLC外标法测定左炔诺孕酮炔雌醚片含量[J].中国现代药物应用,2009,3(2):43-44. |
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| 作者姓名： | 简淑娟 |
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| 作者单位： | 广东省惠州市药品检验所,516001 |
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| 摘 要： | 目的建立外标法测定左炔诺孕酮炔雌醚片含量的方法。方法采用HPLC外标法,以C18柱（150mm×5mm,5μm）为固定相,乙腈-水（80：20）为流动相,检测波长为220nm。结果左炔诺孕酮在0.2418～2.4175μg（r=0.9999）范围内线性关系良好,平均回收率为98.8%,RSD为1.0%（n=9）;炔雌醚在0.1232～1.2316μg（r=1.000）范围内线性关系良好,平均回收率为99.2%,RSD为1.1%（n=9）。结论本方法简便准确,重复性好,可用于控制其内在质量。
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| 关 键 词： | 左炔诺孕酮炔雌醚片 外标法 含量 |
Determination of content of levonorgestrel and quinestrol tablets by HPLC |
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| JIAN Shu-juan.Determination of content of levonorgestrel and quinestrol tablets by HPLC[J].Chinese Journal of Modern Drug Application,2009,3(2):43-44. |
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| Authors: | JIAN Shu-juan |
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| Affiliation: | JIAN Shu-juan.( Huizhou Institute for Drug Control ,Huizhou City, Guangdong 516001, China ) |
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| Abstract: | Objective To establish a HPLC method for determination of content of Levonorgestrel and Quinestrol Tablets. Method C18 column （ 150 mm × 5 mm, 5 μm）was used with a mobile phase of acetonitrilewater（80：20）. The content was detected at wavelength of 220 nm. Result The Levonorgestrel calibration curves were linear in the range of 0. 2418 - 2. 4175 μg（ r = 0. 9999 ）. The average recovery was 98.8% with RSD 1.0% （ n = 9 ）. The Quinestrol calibration curves were linear in the range of 0. 1232 - 1. 2316 μg（ r = 1. 000）. The average recovery was 99. 2% with RSD 1.1% （n =9）. Conclusion The method is simple,realizable and reproducible and can be effectively used for quality control of Levonorgestrel and Quinestrol Tablets. |
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| Keywords: | Levonorgestrel and quinestrol tablets HPLC Content |
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