pGH基因在F_1代转基因金鱼中的整合及表达的分析

本实验对转基因金鱼进行分子生物学鉴定,所用实验材料转基因金鱼是采用显微注射的方法,将羊金属硫蛋白基因启动子与猪生长激素基因(pGH)重组而成的线形DNA片段导入金鱼的受精卵中,在合适的条件下培养获得的。为了鉴定外源基因在金鱼体内整合、表达和遗传的情况,我们选择了相同生长期的F_1代转基因金鱼6条和普通金鱼2条,进行总DNA的提取和纯化,用特异引物对pGH基因进行PCR扩增,结果表明:外源基因(pGH)在部分受体金鱼中得到了整合,并通过有性繁殖稳定地遗传给下一代。同时将阳性金鱼与阴性金鱼进行身长和体重等形态上的比较,初步断定:pGH基因在部分受体鱼中得到了表达,且有显著的促生长作用。

Analysis of Integration and Expression of pGH Gene in F1-Generation Transgenic Goldfish

A linear recombinant plasmid, consisting of the promoter of sheep metallothionein gene and porcine growth hormone (pGH) gene,was rnicroinjected into goldfish fertilized eggs. This study is to detect the experimental goldfish (G1 generation of transgenic goldfish and normal goldfish) using PCR to be sure if the foreign gene (pGH) has been integrated into genome of transgenic goldfish. Our eata shows that the foreign gene has been integrated into genome of some transgenic goldfish, and is passed into next generation through sexual reproduction. Comparing the transgenic goldfish, which contain pGH gene with the others in length and heavy lead to the conclusion that the pGH gene has expressed in some transgenic goldfish and takes a important role in goldfish growth.