Antimicrobial and antioxidant activities of different propolis samples from north‐western Algeria |
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| Authors: | Mokhtar Benhanifia Wessam M. Mohamed Yuva Bellik Hama Benbarek |
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| Affiliation: | 1. Laboratory of Research on Local Animal Products, Institute of Veterinary Sciences, University of Ibn Khaldoun, , Tiaret, 14000 Algeria;2. Graduate School of Pure and Applied Sciences, University of Tsukuba, , Tsukuba, Ibaraki, 305‐8573 Japan;3. Department of Agricultural Sciences, Faculty of life and natural Sciences, University of Mascara, , 29000 Algeria |
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| Abstract: | The aim of this study was the investigation of antimicrobial and antioxidant activities of different ethanolic extracts of propolis (EEP) collected from north‐western Algeria. Total polyphenols and flavonoids contents of extracts were measured. The UV absorption spectrum showed and confirmed their polyphenols constituents. All EEPs exerted antibacterial activity against Gram‐positive bacteria, but no effect on Gram‐negative bacteria. The minimum inhibitory concentration (MIC) of EEPs ranged from 0.01% to 2.6% v/v. The antioxidant activity was measured using ferric‐reducing power (FRAP), 1,1‐diphenyl‐2‐picrylhydrazyl (DPPH) free radical‐scavenging and ESR methods. Propolis TIA‐2 and MOH‐MAS samples showed the highest antioxidative capacity, after 35 min, while TIA‐1, NED‐TL and SFS‐SBA samples showed the highest antioxidative potential of measured EEPs, after 3 min. TIA‐2 sample showed the highest antibacterial, antioxidant activity and highest DPPH free radical‐scavenging activity as well as the highest polyphenols and flavonoids content, compared with other propolis samples. |
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| Keywords: | 2,2‐diphenyl‐1‐picrylhydrazyl Algerian propolis antibacterial activity antioxidants electron spin resonance |
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