| 低聚壳聚糖的复合酶法制备研究 |
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| 引用本文： | 谢华飞,张翠荣,贾振宇,黄金辉.低聚壳聚糖的复合酶法制备研究[J].化学试剂,2012,34(2):125-128,136. |
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| 作者姓名： | 谢华飞 张翠荣 贾振宇 黄金辉 |
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| 作者单位： | 仲恺农业工程学院绿色化工研究所,广东广州,510225 |
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| 基金项目： | 国家自然科学基金，广东省科技攻关资助项目 |
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| 摘 要： | 通过对黏均分子量、酶活力、水解率和还原糖浓度的测定,研究了由商业α-淀粉酶、纤维素酶和果胶酶1∶1∶1(m/m/m)组成的复合酶降解壳聚糖的最佳工艺条件,结果表明:复合酶在酶底物比为1∶5(m/m)、pH 5.3、温度56℃的条件下,酶解2 h可得到分子量为1 000～4 000的低聚壳聚糖,且通过傅里叶红外光谱分析酶解后的产物结构无明显变化。
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| 关 键 词： | 壳聚糖 低聚壳聚糖 复合酶 制备 |
Preparation of low molecular weight chitosan by complex enzymes hydrolysis |
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| XIE Hua-fei
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ZHANG Cui-rong
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JIA Zhen-yu
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HUANG Jin-hui.Preparation of low molecular weight chitosan by complex enzymes hydrolysis[J].Chemical Reagents,2012,34(2):125-128,136. |
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| Authors: | XIE Hua-fei ZHANG Cui-rong JIA Zhen-yu HUANG Jin-hui |
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| Affiliation: | (Institute of Green Chemical Engineering,Zhongkai University of Agriculture and Engineering,Guangzhou 510225,China) |
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| Abstract: | Through determination the viscosity average molecular weight,enzyme activity,hydrolysis ratio and reducing sugar concentration,the best technological conditions of complex enzymes be made up of α-amylase,cellulase and pectinase were investigated.The optimal temperature,pH and ratio of chitosan to complex enzymes are 56 ℃,5.3 and 5∶1,respectively.Low molecular weight chitosan with molecular weight from 1 000 to 4 000 were obtained after chitosan is degradated by the complex enzymes for 2 h,and the structure of enzymatic hydrolysis product was not obviously changes. |
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| Keywords: | chitosan low molecular weight chitosan complex enzymes preparation |
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