Berberine ameliorates the neurological dysfunction of the gastric fundus by promoting calcium channels dependent release of ACh in STZ-induced diabetic rats期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

Berberine ameliorates the neurological dysfunction of the gastric fundus by promoting calcium channels dependent release of ACh in STZ-induced diabetic rats

Affiliation:	1. College of Pharmacy, Hebei Medical University, No. 361 Zhongshan East Road, Shijiazhuang 050017, PR China;2. Beijing Shouyi Group Co., Ltd. Mine Hospital, Tangshan 064400, PR China

Abstract:	BackgroundIt has been reported diabetic gastroparesis is related to diabetic autonomic neuropathy of the gastrointestinal tract, and berberine (BBR) could ameliorate diabetic central and peripheral neuropathy. However, the influence of BBR on the function and motility of the gastric fundus nerve is unclear.MethodsA diabetic rat model was constructed, and HE staining was used to observe the morphological changes in the gastric fundus. The changes in cholinergic and nitrogen-related neurochemical indexes and the effects of BBR on them were measured using Elisa. The effects of BBR on the neural function and motility of gastric fundus were investigated by electric field stimulation (EFS) induced neurogenic response in vitro.ResultsIn the early stage of STZ-induced diabetic rats, the contractile response of gastric fundus induced by EFS was disorder, disturbance of contraction amplitude, and the cell bodies of neurons in the myenteric plexus of gastric fundus presented vacuolar lesions. Administration with BBR could improve the above symptoms. BBR further enhanced the contraction response in the presence of a NOS inhibitor or the case of inhibitory neurotransmitters removal. Interestingly, the activity of ACh could affect NO release directly and the enhancement of BBR on contractile response was canceled by calcium channel blockers completely.ConclusionsIn the early stage of STZ-induced diabetic rats, the neurogenic contractile response disorder of the gastric fundus is mainly related to cholinergic and nitrergic nerve dysfunction. BBR promotes the release of ACh mainly by affecting the calcium channel to improve the neurological dysfunction of the gastric fundus.

Keywords:	Berberine Contractile function Gastric fundus ACh NO Calcium channel BBR"} {"#name":"keyword" "$":{"id":"k0040"} "$$":[{"#name":"text" "_":"Berberine EFS"} {"#name":"keyword" "$":{"id":"k0050"} "$$":[{"#name":"text" "_":"Electrical field stimulation NO"} {"#name":"keyword" "$":{"id":"k0070"} "$$":[{"#name":"text" "_":"Nitric oxide NOS"} {"#name":"keyword" "$":{"id":"k0080"} "$$":[{"#name":"text" "_":"Nitric oxide Synthase VIP"} {"#name":"keyword" "$":{"id":"k0090"} "$$":[{"#name":"text" "_":"Vasoactive intestinal peptide ATP"} {"#name":"keyword" "$":{"id":"k0100"} "$$":[{"#name":"text" "_":"Adenosine triphosphate STZ"} {"#name":"keyword" "$":{"id":"k0110"} "$$":[{"#name":"text" "_":"Streptozotocin TTX"} {"#name":"keyword" "$":{"id":"k0120"} "$$":[{"#name":"text" "_":"Tetrodotoxin AML"} {"#name":"keyword" "$":{"id":"k0130"} "$$":[{"#name":"text" "_":"Amlodipine besylate ACh"} {"#name":"keyword" "$":{"id":"k0140"} "$$":[{"#name":"text" "_":"Acetylcholine Atr"} {"#name":"keyword" "$":{"id":"k0150"} "$$":[{"#name":"text" "_":"Atropine sulfate α-chy"} {"#name":"keyword" "$":{"id":"k0160"} "$$":[{"#name":"text" "_":"α-chymotrypsin Sur"} {"#name":"keyword" "$":{"id":"k0170"} "$$":[{"#name":"text" "_":"Suramin hexasodium salt CCh"} {"#name":"keyword" "$":{"id":"k0180"} "$$":[{"#name":"text" "_":"Carbachol hydrochloride AChE"} {"#name":"keyword" "$":{"id":"k0200"} "$$":[{"#name":"text" "_":"Acetylcholine esterase
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