| RP-HPLC法测定当归芍药散中芍药苷的含量 |
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| 引用本文： | 陈春水,庞晓华.RP-HPLC法测定当归芍药散中芍药苷的含量[J].中国医药导报,2008,5(13):30-31. |
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| 作者姓名： | 陈春水 庞晓华 |
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| 作者单位： | 广州白云山制药总厂第四制造部,广东,广州,510515 |
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| 摘 要： | 目的：建立测定当归芍药散中芍药苷含量的方法。方法：采用反相高效液相色谱法,色谱柱为ODS Diamon-silTMC18（150mm×4.6mm,5μm）,流动相为甲醇-0.5%醋酸（30∶70）,检测波长为230nm,流速为1.0ml/min。结果：芍药苷在20～100μg/ml浓度范围内有良好的线性关系（r=0.9996）;平均回收率为（99.9±1.6）%,RSD为1.14%。结论：本法测定当归芍药散中芍药苷的含量,结果准确、可靠。
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| 关 键 词： | 反相高效液相色谱法 当归芍药散 芍药苷 |
| 文章编号： | 1673-7210(2008)05(a)-030-02 |
| 修稿时间： | 2008年3月13日 |
RP-HPLC method for the content determination of paeoniflorin in Danggui Shaoyao San |
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| CHEN Chun-shui,PANG Xiao-hua.RP-HPLC method for the content determination of paeoniflorin in Danggui Shaoyao San[J].China Medical Herald,2008,5(13):30-31. |
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| Authors: | CHEN Chun-shui PANG Xiao-hua |
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| Affiliation: | (The Fourth Manufacture Department,Guangzhou Baiyunshan Pharmaceutical General Factory, Guangzhou 510515, China) |
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| Abstract: | Objective：To establish the method for the content determination of paeoniflorin in Danggui Shaoyao San. Methods： External standard method was conducted with the column of C18 （150 mm×4.6 mm,5 μm）, the mobile phase was methanol-0.5% acetic acid（30：70）, the flow rate was 1.0 ml/min, and the detective wavelength was set at 230 nm. Results： There was a good linearity in the range of 20-100 μg/ml （r=0.999 6）;the average recovery was （99.9±1.6）% with RSD of 1.14%.Conclusion： The assaying method of RP-HPLC is convenient, accurate, and reliable with good precision, and can be used in the quality control of Danggui Shaoyao San. |
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| Keywords: | RP-HPLC Danggui Shaoyao San Paeoniflorin |
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