HPLC法测定蒲元胃康胶囊中延胡索乙素的含量

目的:建立以高效液相色谱法测定蒲元胃康胶囊中延胡索乙素含量的方法。方法:色谱柱为Venusil XBP-C18(150mm×4.6mm,5μm),流动相为甲醇-0.1%磷酸溶液=60∶40(三乙胺调pH6.0),检测波长为280nm,流速为1mL·min-1,柱温为25℃。以外标法测定含量。结果:延胡索乙素的检测浓度在2.5～100μg·mL-1范围内与峰面积积分值呈良好线性关系(r=0.9996);平均回收率为94.33%,RSD=1.11%(n=6)。结论:本法简便、快速、准确,可用于蒲元胃康胶囊的质量控制。

Determination of Tetrahydropalmatine in Puyuan Weikang Capsule by HPLC

OBJECTIVE： To establish an HPLC method for the determination of the concentration of tetrahydropalmatine in Puyuan weikang capsule. METHODS： The HPLC separation was performed on Venusil XBP-C18 chromatographic column （150 mm×6.0 mm, 5μm） with mobile phase consisted of methanol 0.1% phosphoric acid solution（60 ： 40, with pH adjusted to 6.0 using triethylamine） with a flow rate of 1 mL·min^-1. The detective wavelength was set at 280 nm and the column temperature was set at 25 ℃. RESULTS ： The linear range of tetrahydropalmatine was 2.5-- 100μg· mL^-1 （ r = 0.999 6） and the recovery rate was 94.33% （RSD = 1.11%, n = 6）. CONCLUSION ： The method developed in the study was proved to be simple, rapid and accurate, and applicable for the quality control of Puyuan weikang capsule.