| UV、LiCl复合诱变深黄被孢霉选育γ-亚麻酸突变株 |
| |
| 引用本文： | 魏娜,李柏林,欧杰,蒋志斌.UV、LiCl复合诱变深黄被孢霉选育γ-亚麻酸突变株[J].食品科学,2006,27(12):201-203. |
| |
| 作者姓名： | 魏娜 李柏林 欧杰 蒋志斌 |
| |
| 作者单位： | 上海水产大学食品学院,上海,200090 |
| |
| 基金项目： | 上海市重点学科建设项目（T1102） |
| |
| 摘 要： | 以深黄被孢霉（Mortierella isabellina）AS3．3410为出发菌株，经UV、LiCl复合诱变处理，随机筛选后复筛，最终获得一株突变株SHFU-13。其种子发酵2d，摇瓶发酵8d时，生物量和总脂量分别达21．66、12．65g/L，在同等条件下比出发菌株分别提高了8．84％和36．61％。气相色谱分析其油脂组成，γ-亚麻酸含量为6．39％，产量为0．81g／L，产量较对照样提高44．64％。连续传代多次，其产量性状无显著变化。
|
| 关 键 词： | 深黄被孢霉 γ-亚麻酸 诱变 |
| 文章编号： | 1002-6630（2006）12-0201-03 |
| 收稿时间： | 2006-07-09 |
| 修稿时间： | 2006-07-09 |
Screening of A Producing γ-linolenic Acid Mutant Strain (Mortierella isabellina) by UV and LiCl |
| |
| WEI Na,LI Bai-lin,OU Jie,JIANG Zhi-bin.Screening of A Producing γ-linolenic Acid Mutant Strain (Mortierella isabellina) by UV and LiCl[J].Food Science,2006,27(12):201-203. |
| |
| Authors: | WEI Na LI Bai-lin OU Jie JIANG Zhi-bin |
| |
| Affiliation: | College of Food Science, Shanghai Fisheries University, Shanghai 200090, China |
| |
| Abstract: | A mutant strain SHFU-13 was successfully obtained when the original strain Morrierella isabeUina AS3.3410 was treated by UV and LiCl as mutating agent. The biomass was up to 21.66g/L, which was 8.84% higher than that of the original strain while the product of the total lipid was up to 12.65g/L, which was 36.61% higher than that in the original strain mycelium. Analysis by gas chromatograph indicated the content of GLA in the lipid was 6.39% while the product of it was up to 0.81g/L, which was 44.64% higher than that of the original strain. After passing on many times, there were no distinct changes on the production. |
| |
| Keywords: | Mortierella isabellina GLA mutation |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《食品科学》浏览原始摘要信息 |
|
点击此处可从《食品科学》下载全文 |
