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肌动蛋白是大鼠肝细胞核基质的组分之一
引用本文:李璟,温博贵,吴健谊,陈爱云.肌动蛋白是大鼠肝细胞核基质的组分之一[J].汕头大学医学院学报,2001,14(1):13-14,16.
作者姓名:李璟  温博贵  吴健谊  陈爱云
作者单位:汕头大学医学院生物化学教研室
基金项目:中国预防医学科学院立项课题
摘    要:目的:证实肌动蛋白存在于大鼠肝细胞核基质中。方法:应用SDS-PAGE法分析大鼠肝细胞核基质的组分,以肌动蛋白多克隆抗体进行Westem Blot免疫印迹检测。结果:核基质蛋白SDS-PAGE图谱中可见相当于肌动蛋白的43KD条带,Westem Blot分析证实核基质中存在肌动 蛋白条带。结论:肌动蛋白是大鼠肝细胞核基质的组分之一。

关 键 词:肌动蛋白  大鼠  肝细胞  核基质  SDS-PAGE法
文章编号:1007-4716(2001)01-0013-03

Actin is a Component of the Nuclear Matrices of Rat Hepatocytes
Li Jing,Wen Bogui,Wu Jianyi,Chen Aiyun.Actin is a Component of the Nuclear Matrices of Rat Hepatocytes[J].Journal of Shantou University Medical College,2001,14(1):13-14,16.
Authors:Li Jing  Wen Bogui  Wu Jianyi  Chen Aiyun
Abstract:Objective:To determine actin in the nuclear matrices of the hepatocytes of rats.Methods:The nuclear matrices of hepatocytes were analyzed by SDS_PAGE and the actin was detected by Western Blot.Results:The polypeptide of 43 KD was found to occur in the nuclear matrices of the hepatocytes.It was determined to be actin by Western Blot analysis.Conclusion:The actin was demonstrated to be a component of the nuclear matrices of hepatocytes of rats.
Keywords:Actin  Rat  Hepatocyte  Nuclear Matrix
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