591株结核分枝杆菌耐药情况分析

目的回顾性分析湖南省结核病医院结核病人中结核分枝杆菌耐药状况并分析耐药形成原因。方法分析2003年5月1日—2007年12月31日间分离培养出并使用噻吩-2羧基肼（TCH）和对硝基苯甲酸（PNB）进行分型确认的591株结核分枝杆菌药物敏感试验结果，包括异烟肼（INH、H）、利福平(RFP、R)、硫酸链霉素（SM、S）、对氨基水杨酸钠(PAS、P)、乙胺丁醇(EMB、E)、卡那霉素(KM、K)、左氧氟沙星（OFLX、O）。7种药物采用绝对浓度法进行药物敏感性检测。结果591株结核分枝杆菌中，至少耐一药为63.6%（376/591）；同时对7种药物耐药率为0.2%（1/591）；耐多药率（MDR）为38.6%(228/591)。结论结核病人耐药情况比较严重，需要更严格的执行结核病控制策略，防止耐药病人及耐药菌株的产生和传播。

Investigation on the drug resistance status of 591 strains Mycobacterium tuberculosis

Objective To analyze retrospectively the status of drug resistance of mycobacterium tuberculosis（MTB） isolated from patients hospitalized in Hunan tuberculosis hospital, and to ana- lyze the causes. Methods We analyzed the results of drug susceptibility testing in 591 strains MTB isolated from May 1,2003 to December 31,2007. The drug susceptibility of all isolates were detected by absolute concentration method among 7 drugs, including INH （H）, RFP（R）, SM （S）, PAS （P）, EMB （E）,KM （K）,OFLX（O）. Results Of 591 isolates,376（63.6%） isolates were re- sistant to one drug at least. 1（0. 2%） isolates was resistant to all seven drugs. 228（38.6%） were MDR isolates. Conclusions The situation of drug resistance is very serious. Strategies should be stringently taken to prevent and treat patients with drug-resistance pulmonary tuberculosis.