| 双功能抗体介导CIK细胞杀伤胃癌细胞的实验研究 |
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| 引用本文： | 张林,侯艳红,段云卉,李丹.双功能抗体介导CIK细胞杀伤胃癌细胞的实验研究[J].胃肠病学和肝病学杂志,2010,19(11). |
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| 作者姓名： | 张林 侯艳红 段云卉 李丹 |
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| 作者单位： | 解放军第309医院消化科;解放军第309医院门诊部; |
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| 摘 要： | 目的初步探讨抗EGFR/抗CD3双功能抗体介导对于CIK细胞杀伤胃癌细胞MKN45及SGC7901能力的影响。方法通过化学耦联法合成抗EGFR/抗CD3双功能抗体并检验其双特异抗体功能。采用免疫细胞化学法明确靶细胞的EGFR表达情况。通过细胞结合率检测及MTT杀伤实验检测抗EGFR/抗CD3双功能抗体介导CIK细胞与胃癌细胞结合及杀伤的能力,并与单纯CIK细胞、单纯EGFR单抗和/或CD3单抗介导CIK细胞的结合及杀伤作用比较。结果抗EGFR/抗CD3双功能抗体介导CIK细胞与胃癌细胞株MKN45及SGC7901结合率分别为(64.6±7.2)%和(59.5±6.1)%,显著高于其余各对照组(P0.05),对照组间无显著性差异;MTT细胞杀伤实验结果提示:抗EGFR/抗CD3双功能抗体介导CIK细胞对胃癌细胞株杀伤率显著高于单纯抗CD3单抗介导及抗CD3单抗联合抗EGFR单抗介导组,而后两组杀伤率又显著高于单纯抗EGFR单抗介导组及单纯CIK细胞组(P0.05)。结论体外实验初步显示抗EGFR/抗CD3双功能抗体介导可显著提高CIK细胞的对胃癌细胞的结合及杀伤能力。
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| 关 键 词： | 胃癌 CIK细胞 双功能抗体 |
The cytotoxicity activity of CIK cells against gastric cancer cell lines mediated by bispecific antibody |
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| ZHANG Lin,HOU Yanhong,DUAN Yunhui,LI Dan.The cytotoxicity activity of CIK cells against gastric cancer cell lines mediated by bispecific antibody[J].Chinese Journal of Gastroenterology and Hepatology,2010,19(11). |
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| Authors: | ZHANG Lin HOU Yanhong DUAN Yunhui LI Dan |
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| Affiliation: | ZHANG Lin1,HOU Yanhong2,DUAN Yunhui1,LI Dan1 1. Department of Gastroenterology,2. Department of Out-patient,the 309 Hospital of PLA,Beijing 100091,China |
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| Abstract: | Objective To investigate the cytotoxicity activity of CIK cells targeted by EGFR/CD3 bispecific antibody ( BsAb) against gastric cancer line SGC7901 and MKN45. Methods A bispecific antibody was constructed by chemically crosslinking one monoclone antibody against human CD3 and one monoclone antibody ( McAb) against human EGFR. The immunocytochemistry assay was performed to detect the expression of EGFR in SGC7901 and MKN45 cell lines. The cytotoxicity activity of CIK cells targeted by EGFR/CD3 BsAb was anal... |
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| Keywords: | Gastric cancer CIK cell Bispecific antibody |
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