气滞血瘀证大鼠NO/NOS体系的变化

目的：观察气滞血瘀证大鼠一氧化氮／一氧化氮合成酶（NO／NOS）的体系变化。方法：将Wistar大鼠随机分成4组，对照组、模型组、血府逐瘀汤低、高剂量组。采用多因素联合刺激法建立气滞血瘀模型。运用分光光度法测定血浆和肝组织中NO含量以及肝组织中NOS活性，利用RT—PCR法测定肝组织中NOS基因表达。结果：模型组血浆NO含量（0．52±0．33）μmol／L显著低于对照组（2．77±1．73）μmol／L（P〈0．01），低剂量组NO含量（2．37±0．53）μmol／L显著高于模型组（0．52±0．33）μmol／L（P〈0．01）；模型组肝组织NO含量（0．52±0．24）μmol／mg显著低于对照组（5．87±1．72）μmol／mg（P〈0．01），低剂量组NO含量（3．72±1．53）μmol／mg显著高于模型组（0．52±0．24）μmol／mg（P〈0．01）；模型组肝组织eNOS基因表达水平显著低于对照组（P〈0．01），低剂量组肝组织eNOS显著高于模型组（P〈0．05）。结论：气滞血瘀证大鼠NO／NOS体系下调，为进一步研究治疗血瘀症类疾病提供了借鉴和思路。

Change of NO/NOS in Rats of Qi-blood Stagnation

Objective： To investigate change of nitric oxide （NO）/NO synthase （NOS） in Qi-blood stagnation of rats. Methods ： Wistar rats were randomized to four groups： normal control group, model group, low concentration group, and high concentration group. The Qi-blood stagnation model was established by complex pathogens. The content of NO in plasma and liver tissues of rats was measured by spectrophotometric. RT-PCR was used to analyze liver tissues NOS mRNA expression. Results： The plasma NO of model group （0.52±0.33）μmol/L] decreased significantly compared with normal control group （2.77±1.73）μmol/L] （P 〈0.01 ）. The plasma NO of treatment group （ 2.37±0.53 ）μmol/L ] increased significantly compared with model group （ 0.52±0.33 ） μmol/L ] （ P 〈 0.01 ）. The NO in liver tissues of model group （0.52±0.24 ） μmol/mg ] decreased significantly compared with normal control group （5.87 ± 1. 72 ） μmol/mg ] （ P 〈 0.01 ）. The NO in liver tissues of treatment group （ 3.72 ±1.53 ） μmol/mg ] increased significantly compared with model group （0.52±0.24） μmoL/mg] （P 〈0.01 ）. The expression of NOS in liver tissues NOS mRNA expression in model group de-creased significantly compared with normal control group （P 〈 0.01 ）. The expression of NOS in liver tissues NOS mRNA expression in low concentration increased significantly compared with model group （P 〈0.05 ）. Conclusion： Nitric oxide（ NO）/NO synthase（NOS） was down-regulated in Qi-blood stagnation rats.