Sulfatide-tenascin interaction mediates binding to the extracellular matrix and endocytic uptake of liposomes in glioma cells |
| |
Authors: | K Shao Q Hou M L Go W Duan N S Cheung S -S Feng K P Wong A Yoram W Zhang Z Huang Q -T Li |
| |
Affiliation: | (1) Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, 21 Lower Kent Ridge Road, Singapore, 119077, Singapore;(2) Department of Pharmacy, Faculty of Science, National University of Singapore, Singapore, Singapore;(3) Institute of Biotechnology, Deakin University, Victoria, 3217, Australia;(4) Department of Chemical and Biomolecular Engineering, Faculty of Engineering, National University of Singapore, Singapore, Singapore;(5) Department of Pharmacology, School of Pharmacy, Faculty of Medicine, Hebrew University of Jerusalem, Jerusalem, 91120, Israel |
| |
Abstract: | Tenascin-C is an extracellular matrix glycoprotein, whose expression is highly restricted in normal adult tissues, but markedly
up-regulated in a range of tumors, and therefore serves as a potential receptor for targeted anticancer drug or gene delivery.
We describe here a liposomal carrier system in which the targeting ligand is sulfatide. Experiments with tenascin-C-expressing
glioma cells demonstrated that binding of liposomes to the extracellular matrix relied essentially on the sulfatide-tenascin-C
interaction. Following binding to the extracellular matrix, the sulfatide-containing liposomes were internalized via both
caveolae/lipid raft- and clathrin-dependent pathways, which would ensure direct cytoplasmic release of the cargoes carried
in the liposomes. Such natural lipid-guided intracellular delivery targeting at the extracellular matrix glycoproteins of
tumor cells thus opens a new direction for development of more effective anticancer chemotherapeutics in future.
K. Shao & Q. Hou: These authors contributed equally to this work.
Received 22 September 2006; received after revision 5 December 2006; accepted 9 January 2007 |
| |
Keywords: | Ligand-targeted drug delivery liposomes sulfatide tenascin-C extracellular matrix |
本文献已被 PubMed SpringerLink 等数据库收录! |
|